肝细胞癌的替代拼接景观及其对HCC检测潜力
Courtney E Hershberger1,2, Noah J Daniels1,2, William M Patterson3
1Department of Quantitative Health Sciences, Cleveland Clinic Research, Cleveland Clinic, Cleveland, Ohio, USA.
Hepatology communications
|December 12, 2025
概括
在肝细胞癌 (HCC) 中可以检测到循环中的替代拼接事件 (ASEs). 循环上皮细胞中的特定ASEs显示出早期HCC检测的希望,特别是当与α-fetoprotein (AFP) 结合时.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 预mRNA替代拼接与肝细胞癌 (HCC) 瘤发生有关.
- 在循环中可以检测到某些致癌拼接异型,这表明潜在的非侵入性生物标志物.
研究的目的:
- 为了全面描述HCC的替代拼接景观.
- 在外周流体中识别特定于HCC的替代拼接事件 (ASEs),以便早期检测.
主要方法:
- 分析了787个HCC瘤样本的RNA-Seq数据,以确定常见的错误拼接ASE.
- 在外周流体中发现了ASEs,包括循环上皮细胞 (CEC),血小板,血清和血.
- 机器学习模型经过训练,使用ASEs在CEC和等离子中对HCC进行分类.
主要成果:
- 在HCC中发现了3416个常见的错误拼接ASEs,并通过HCC-INSIGHT网络应用程序提供.
- 十个CEC的ASE区分HCC与非HCC,准确度为74%.
- 将CEC ASEs与α-fetoprotein (AFP) 结合起来,可以提高分类,达到78%的平衡准确度.
结论:
- 在HCC中,替代拼接事件 (ASEs) 显示出作为循环生物标志物的潜力.
- 在循环上皮细胞 (CEC) 中的ASEs对HCC检测有希望,特别是与AFP结合使用时.
- 需要在潜在队列中进一步验证; HCC-INSIGHT 作为研究资源.
关键词:
替代性拼接是一种替代性的拼接.生物标志物生物标志物它们是前代子.人类 人类 人类 人类 人类 人类 人类内部子 (intron) 是一个内部子.这种异形异形是什么?液体活检活检液体活检机器学习是机器学习.更多相关视频
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