布菲配方在老鼠中的药理动力学,组织分布和新陈代谢的全面描述
Hou-Xu Ma1, Na-Wei Qi1, Xiao-Lin Yang2
1State Key Laboratory of Nanjing Medicines, China Pharmaceutical University, Nanjing 211198, China.
Journal of pharmaceutical and biomedical analysis
|December 12, 2025
概括
这项研究跟踪了老鼠的Bufei配方 (BF),确定了63种化合物,包括17种原始药物和46种代谢物. 肺和结肠中积累的BF成分,有助于了解其治疗作用.
科学领域:
- 药理学 药理学是指药理学的学科.
- 传统中国医药 传统中国医药
- 药理动力学 药理动力学
背景情况:
- 百合配方 (BF) 是一种传统的中医药,用于治疗呼吸系统疾病.
- 它复杂的草药成分阻碍了对其体内机制的理解.
- 阐明BF的药理动力学和新陈代谢对于其临床应用至关重要.
研究的目的:
- 系统地研究大鼠BF的药理动力学,组织分布和代谢特征.
- 为了在体内识别活性成分及其代谢物.
- 为了解BF的药理动力学物质基础提供一个基础.
主要方法:
- 超高性能液体色谱 (UPLC) 用于成分识别.
- 在生物样本 (血,尿液,便,组织) 中识别原型化合物和代谢物的UPLC-tandem质谱学 (UPLC-MS/MS).
- 验证的UPLC-MS/MS方法用于定量14种活性成分.
主要成果:
- 在体内确定了63种成分:17种原型化合物和46种代谢物.
- 量化了14个活性成分,其中8个显示出显著的药理动力学参数 (Tmax,Cmax,AUC).
- 组织分布分析显示分布广泛,在肺和结肠中优先积累.
结论:
- 这项研究提供了BF在老鼠中的综合性药理动力学,分布和代谢数据.
- 确定肺和结肠是BF作用的潜在目标器官.
- 为Bufei Formula的临床使用和机制理解提供了宝贵的见解.
更多相关视频
08:28Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug
Published on: December 14, 2021
9.9K
08:59An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
Published on: December 3, 2020
8.5K
相关概念视频
01:20Measurement of Bioavailability: Pharmacodynamic Methods
212
Pharmacodynamic methods provide insights into a drug's effects on physiological processes over time and play a crucial role in understanding bioavailability and therapeutic efficacy. These methods can be broadly classified into acute pharmacological and therapeutic response approaches, each with distinct mechanisms and applications.The acute pharmacological response method directly correlates a drug's physiological effects, such as ECG or pupil diameter changes, to its time course in the body.
212
01:30Measurement of Bioavailability: Pharmacokinetic Methods
236
Pharmacokinetics is a vital branch of pharmacology that examines how drugs are absorbed, distributed, metabolized, and excreted by the body. Two key methodologies in pharmacokinetics are plasma drug concentration studies and urinary drug excretion analyses, both of which provide critical insights into a drug's therapeutic efficacy and bioavailability.Plasma Drug Concentration-Time StudiesPlasma drug concentration-time studies involve analyzing blood samples at specific intervals to quantify...
236
01:01Dosage Regimens: Partial Pharmacokinetic Parameters
133
It is not uncommon for complete drug pharmacokinetic profiles to remain elusive in pharmacokinetics. This necessitates certain educated assumptions by pharmacokineticists to determine appropriate dosage regimens without comprehensive pharmacokinetic data from animal or human studies. One prevalent assumption is setting the bioavailability factor, denoted as F, to 1 or 100%. This assumption caters to the scenario where a drug doesn't achieve full systemic absorption, resulting in the patient...
133
01:13Physiological Pharmacokinetic Models: Assumption with Protein Binding
196
Physiological models with protein binding in pharmacokinetics offer a sophisticated approach to understanding drug disposition. These models consider drug-protein interactions, enabling them to effectively predict drug concentrations in different organs and tissues. This precision aids in accurate drug dosing, providing a significant advantage over conventional models. A key process within these models is equilibration, which ensures that drug concentrations achieve a steady state within the...
196
01:11Bioavailability Study Design: Single Versus Multiple Dose Studies
179
Bioavailability studies are essential for understanding how a drug is absorbed, distributed, metabolized, and excreted in the body. These studies assess the extent and rate at which the active pharmaceutical agent becomes available at the site of action. The design of bioavailability studies can involve single-dose or multiple-dose regimens, each with distinct advantages and limitations.Single-dose studies are the preferred approach due to their simplicity and reduced drug exposure for...
179
01:27Bioavailability Study Design: Absolute Versus Relative Bioavailability
267
Bioavailability is a crucial pharmacokinetic parameter that quantifies the proportion of an administered drug that reaches the systemic circulation and is available for therapeutic action. Regulatory agencies mandate the assessment of bioavailability, typically measured as the area under the drug plasma concentration-versus-time curve (AUC), to ensure the efficacy and safety of pharmaceutical products. These evaluations are categorized as absolute and relative bioavailability studies.Absolute...
267