菌体诱导的内器效应为敏感的E. coli O157:H7双模式检测提供了强度
Xiaoyong Ma1, Peihao Yuan1, Saqlain Ali Bukhari1
1State Key Laboratory of Utilization of Woody Oil Resource, National Engineering Research Center of Rice and Byproducts Deep Processing, Hunan Key Laboratory of Forestry Edible Resources Safety and Processing, Changsha Technical Innovation Center for Food Functional Ingredients, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
Food chemistry
|December 12, 2025
概括
一种新型的菌体诱导传感器快速检测出大肠杆菌O157:H7.7. 这种双模式系统使用工程M13菌体来增强局部表面等离子体共振 (LSPR) 和内部波器效应 (IFE),用于敏感的食物传播病原体检测.
科学领域:
- 生物感应是一种生物感应.
- 纳米技术纳米技术
- 食品安全 食品安全
背景情况:
- 大肠杆菌O157:H7是一种危险的食物传播病原体,需要快速检测.
- 基于内部波效应 (IFE) 的传感器提供双模式检测,但与局部表面等离子体共振 (LSPR) 调制和信号放大作斗争.
研究的目的:
- 开发一种菌体诱导的IFE双模式传感器,用于增强大肠杆菌O157:H7检测.
- 改进基于IFE的传感器中的LSPR调制和信号放大.
主要方法:
- 工程M13菌体显示大肠杆菌特异性和链胺结合.
- 使用磁珠捕获Aptamer介导的细菌,并自组装Streptavidin涂层黄金纳米粒子 (SA-AuNPs).
- 利用菌体P8终端间距来接近SA-AuNP,增强LSPR和IFE介导的量子点光火.
主要成果:
- 实现了对大肠杆菌O157:H7.7的敏感双模式检测.
- 通过高的P8副本数来证明信号放大,使多个IFE事件成为可能.
- 在食品样本中报告的线性范围为10^2-10^7 CFU/mL,检测极限为3 CFU/mL,以及高回收率 (86.6%-110.6%).
结论:
- 菌体诱导的IFE双模式传感器显示了对大肠杆菌O157:H7检测的高选择性和灵敏性.
- 这种传感器在食品安全测试中具有很强的实际应用潜力.
- 工程菌体系统有效地增强LSPR和IFE,以提高生物传感性能.
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