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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jan 8, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

转录引导的向细胞丰富用于可扩展的单核RNA测序.

Andrew Liao¹, Zehao Zhang², Andras Sziraki²

¹Laboratory of Single-Cell Genomics and Population Dynamics, the Rockefeller University, New York, NY, USA; The Tri-Institutional MD-PhD Program, New York, NY, USA.

|December 12, 2025

概括

此摘要是机器生成的。

EnrichSci是一种新方法,用于分析衰老组织中的罕见细胞类型. 它揭示了老化特异性的基因表达和转录后的变化在寡类细胞,提供了更深入的洞察细胞动态.

关键词:

大脑衰老老龄化目标细胞类型目标细胞类型综合性索引是指指数的组合方式.在外动力学方面,外动力学杂交的连锁反应是混合的.异形切换的切换方式类质细胞 (Oligodendrocytes) 是一种有机细胞.一个单细胞RNA测序.拼接拼接拼接拼接转录组的动力学

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

相关实验视频

Last Updated: Jan 8, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

科学领域:

分子生物学分子生物学
神经科学是一个神经科学.
基因组学就是基因组学.

背景情况:

单细胞地图集识别了与衰老和疾病相关的细胞类型.
这些细胞类型在组织中通常代表性不足,阻碍了分析.
对稀有细胞进行详细的分子分析对于了解衰老和疾病至关重要.

研究的目的:

开发一个可扩展的平台,用于分析复杂组织中代表性不足的细胞类型.
为了研究与衰老相关的分子动力学,在寡头细胞亚型.
为了在外子水平上识别新的衰老特征.

主要方法:

开发了EnrichSci,这是一个无微流体的平台,将杂交连锁反应RNA光 in situ杂交 (FISH) 与组合索引相结合.
应用EnrichSci对老化小鼠大脑中的寡核细胞单核转录组进行了分析.
分析了基因水平和外子水平的表达变化.

主要成果:

通过EnrichSci,可以对目标细胞类型进行可扩展的分析,并完全覆盖基因体.
发现了与衰老相关的分子动力学,在不同类型的寡细胞亚型中.
鉴定了与衰老相关的异子级特征,这些特征在传统的基因级分析中被遗漏,这表明了转录后调节的重要性.

结论:

EnrichSci是一种多功能方法,用于从复杂组织中解码来自复杂组织的多种细胞类型的动态调节场景.
转录后调节在衰老期间的细胞状态动态中起着至关重要的作用.
该平台为代表性不足的细胞群的详细分子分析提供了便利.