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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
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Photoreceptors and Visual Pathways

At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category, whereas...

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相关实验视频

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通过度依赖的光来成像线粒体膜潜力,改变了整个生命周期的变化.

Dilizhatai Saimi1, Luc Reymond2, Tursunjan Aziz3

  • 1College of Future Technology, Institute of Molecular Medicine, National Biomedical Imaging Center, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Peking University, Beijing, China.

Nature communications
|December 12, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的线粒体探针PK Mito Deep Red (PKMDR). 它的光寿命准确地测量了线粒体膜潜力 (Δψm),揭示了细胞和组织中的代谢异质性.

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科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 生物物理学的生物物理.

背景情况:

  • 线粒体对于细胞代谢和能量生产至关重要.
  • 目前用于线粒体成像的光工具在空间时间分辨率方面存在局限性.
  • 需要直接报告线粒体状态,特别是膜潜力.

研究的目的:

  • 介绍PK Mito Deep Red (PKMDR),一种用于线粒体成像的新型光探针.
  • 确定PKMDR的光寿命作为线粒体膜潜力的敏感指标 (Δψm).
  • 证明基于PKMDR的光终身成像显微镜 (FLIM) 对可视化代谢异质性的实用性.

主要方法:

  • 开发和描述PKMDR,一个低光毒性的线粒体探针.
  • 使用光终身成像显微镜 (FLIM) 来测量 Δψm.
  • 在活细胞,有机体和组织中应用PKMDR-FLIM,用于时差成像.

主要成果:

  • PKMDR光寿命是 Δψm 的敏感指标,与线粒体呼吸和氧化酸化相关.
  • 在单个细胞,有机体和组织中,PKMDR-FLIM有效地可视化异质的Δψm.
  • 该方法揭示了外周线粒体和周核线粒体之间的代谢差异.

结论:

  • PKMDR是一种强大的光探针,用于评估线粒体膜潜力.
  • PKMDR-FLIM提供高时空分辨率可用于可视化线粒体代谢状态.
  • 这种技术为细胞和组织的代谢异质性提供了新的见解.