一个明亮的和再生的阵列标签与化连接体的长期活细胞纳米镜和单分子跟踪
Dongjuan Si1, Zhihao Dong1, Gang Xing1
1MOE Key Laboratory of Smart Drug Delivery, MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases, School of Pharmacy, Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
Nature communications
|December 12, 2025
概括
研究人员开发了ReTagX,这是一种用于活细胞成像的新型光标签系统. 这种再生系统克服了光漂白,使得长期,高分辨率可视化细胞动态.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 显微镜的使用方法
背景情况:
- 活细胞纳米镜和单分子追踪对细胞生物学至关重要.
- 目前的光标签遭受低亮度,大尺寸和光漂白,限制应用.
研究的目的:
- 为了介绍ReTagX,一个明亮的,再生的,紧的阵列标签系统.
- 通过可逆的化标签来实现光漂白信号的快速恢复,以改善活细胞成像.
主要方法:
- 开发ReTag1 (~18kDa) 与MaP655-TMP用于高性.
- 利用ReTagX进行线粒体TOM20的刺激发射枯竭 (STED) 成像.
- 应用 ReTagX 阵列 (例如,ReTag8,~156 kDa) 用于单分子追踪 TNFR2.2.
主要成果:
- ReTag1实现了高达204倍的信号噪声比,在1分钟内恢复光.
- 扩展了线粒体动态的STED成像,以80nm分辨率超过100分钟.
- 多重 ReTag1 阵列增加了 5.5 倍的亮度,并扩展了 TNFR2 追踪 ~ 2.9 倍.
结论:
- ReTagX提供了一个强大的平台,用于在活细胞中进行高分辨率的长期分子可视化.
- 该系统克服了传统光标签的局限性,增强了细胞动态研究.
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