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在使用ddRAD测序的本土红色辛迪牛的拷贝数变异的全基因组检测

Sonali Sonejita Nayak¹, Shikha Mittal², Surya Kant Verma¹

¹Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.

Mammalian genome : official journal of the International Mammalian Genome Society

|December 13, 2025

概括

这项研究使用ddRAD-seq绘制了红辛迪牛的副本数变异 (CNVs),识别了抗病和耐热性关键基因. 研究结果支持气候智能牲畜的标记器辅助选择.

科学领域:

基因组学就是基因组学.
动物遗传学动物遗传学
生物信息学是一种生物信息学.

背景情况:

副本数变异 (CNVs) 是影响牲畜特征的关键结构基因组差异.
红色辛迪牛是土著的,因其抗病性和耐热性而受到重视.
了解这种品种的CNV可以提高适应性和生产力.

研究的目的:

为了对红色辛迪牛的全基因组CNV进行表征.
识别与适应性特征相关的基因和定量特征位点 (QTLs).
为这个本土品种建立一个全面的CNV地图.

主要方法:

双消化限制部位相关的DNA测序 (ddRAD-seq) 用于全基因组分析.
使用CNVnator进行了CNV识别,其容量为1000bp.
功能注释使用PANTHER和Animal QTLdb进行基因和QTL关联.

主要成果:

总共有3,269个高可信度的CNV被确定,主要是在自体上.
在1号和18号染色体上发现了显著的CNV,包括重复和删除.
CNV重叠了2593个与112个QTLs相关的基因,影响了适应性,免疫力,乳生产和生殖.

结论:

这项研究提供了第一个全面的红辛迪牛CNV地图.
ddRAD-seq是一个负担得起的工具,用于本地品种的基因组研究.
这些发现支持气候智能热带牲畜的标记器辅助选择和保护.

关键词:

适应适应适应副本数量的变化副本数量的变化基因组多样性基因组多样性红色的辛迪牛牛.结构变体结构变体在 ddRAD-seqq 中.

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相关概念视频

Comparing Copy Number Variations and SNPs

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...