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在大米害虫Chilo suppressalis中的非逆转录病毒内源性病毒元素的全面表征

Jia-Bao Lu¹, Yu-Hua Qi¹, Ying Tian¹

¹State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

|December 13, 2025

概括

这项研究在大米干的基因组中确定了15种非逆转录病毒内源性病毒元素 (nrEVEs),揭示了它们的起源和病毒与宿主共同进化的潜在作用. 这些发现促进了对基因组结构和长期病毒相互作用的理解.

科学领域:

病毒学病毒学
基因组学就是基因组学.
昆虫生物学昆虫生物学

背景情况:

非逆转病毒内源性病毒元素 (nrEVEs) 是分子化石,为真核生物,特别是昆虫的病毒宿主共同进化提供了洞察力.
条纹大米干 (Chilo suppressalis) 是一个重要的农业害虫,分布广泛.

研究的目的:

为了全面地描述nREVEs在Chilos suppressalis.
研究这种昆虫物种中的nrEVEs的起源,整合模式和表达.

主要方法:

将元转录组和小RNA (sRNA) 测序数据与现有的基因组数据集成.
生物信息分析,包括遗传学分析和转录基因分析.
识别与集成相关的领域和主机转录协会.

主要成果:

在C. suppressalis基因组中发现了15个nrEVEs (13个来自Orthomyxoviridae,2个来自Rhabdoviridae).
nrEVEs表现出不同的整合方向,已知的病毒的序列身份,以及特定的表达模式 (发育,组织,地理).
在宿主转录中发现了12个nrEVEs,其中有3个是分子养的潜在候选者;检测到大量的piRNA类读数.

结论:

这项研究提供了C. suppressalis中nrEVEs的全面表征,突出了它们与宿主mRNA的关联.
这些发现有助于我们更好地理解这种害虫物种的长期病毒宿主共同进化和基因组结构形成.
为未来研究nrEVEs的功能作用奠定了基础.

关键词:

奇洛 suppressalis 的使用情况.它们属于Orthomyxoviridae.拉巴多维里达 (Rhabdovirida) 是一个夸兰贾病毒病毒.没有REVEs.病毒宿主共同进化这是一种像piRNA一样的piRNA.

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