来自Pyrolobus fumarii的热稳定III类基酸盐合成酶1A:基酸盐生物合成的特征和应用潜力
Jingfei Wu1, Takanori Akiyama2, Yasuhisa Asano1
1Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.
Chembiochem : a European journal of chemical biology
|December 13, 2025
概括
我们从高热友的古生物群中鉴定出一种热稳定的基酸盐合成酶 (PfPRS). 这种酶具有高度稳定性和效率,在生物合成中显示出工业应用的潜力.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 极端动物生物学 极端动物生物学
背景情况:
- 基酸 (PRPP) 是合成核酸,氨基酸和辅助因子的关键代谢中间体.
- 超热友的古生物拥有适应极端温度的独特酶,为生物技术应用提供了潜力.
- 了解PRPP合成酶的功能和稳定性是优化生物合成途径的关键.
研究的目的:
- 鉴定和描述来自超热友的古生物Pyrolobus fumarii 1A.的热稳固基酸盐合成酶 (PfPRS).
- 评估酶的催化活性,热稳定性和基质特异性.
- 探索使用PfPRS用于PRPP生物合成的可行性,包括联合固定化策略.
主要方法:
- 基因鉴定和prs的异质表达在大肠杆菌.
- 再组合PfPRS的净化和生化表征,包括pH和最佳温度.
- 热稳定性测试,运动分析 (Km,kcat) 和与多酸盐激酶2 (DrPPK2) 联合固定.
主要成果:
- 在pH 7.5和55°C时,PfPRS表现出最佳活性,在广泛的pH范围 (4.0-10.5) 中保持了超过85%的活性.
- 该酶表现出了显著的热稳定性,其半衰期在90°C时为12h,在95°C时为5h,在100°C时为3h.
- PfPRS 显示出 ATP 作为二酸盐供体的偏好,并成功与 DrPPK2 共同固定,以实现潜在的 ATP 再生.
结论:
- 发现的PfPRS是一种高度热稳定和高效的酶,具有生物技术应用的巨大潜力.
- 在极端条件下的稳定性使其成为工业PRPP生产的有价值候选者.
- 协同固定化策略显示出开发生物合成的综合酶系统的前景.
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