贾斯莫纳特通过激活SnRK2.1来调节WRKY50-JAZ5模块来调节草对人类的敏感性
Chuang Liu1, Zhen Liu1, Xia Li1
1Department of Pomology, College of Horticulture, China Agricultural University, Beijing, China.
Plant biotechnology journal
|December 13, 2025
概括
甲基斯酸盐 (MeJA) 通过激活JA生物合成反循环,增加了草对炭糖的敏感性. 准FveWRKY50和FveJAZ5基因可以创建抗疾病的草品种.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 植物与微生物的相互作用
背景情况:
- 科莱托里菌 (Colletotrichum fungi) 导致炭菌,这对全球草产量构成重大威胁.
- 众所周知,斯酸盐 (JA) 信号通路调节了植物病原体相互作用,但它们在草中扮演的特定角色在很大程度上仍未被定义.
研究的目的:
- 阐明斯酸盐 (JA) 信号影响草对科莱托里奇姆病原体的易感性的分子机制.
- 为了确定关键的基因和调节途径,涉及到植物对炭糖的反应.
主要方法:
- 用外源甲基斯酸盐 (MeJA) 对草植物的处理和内源MeJA水平的分析.
- 对JA生物合成基因 (FveAOS2,FveAOC3) 和调控组件 (FveSnRK2.1,FveWRKY50,FveJAZ5) 的鉴定和表征.
- 研究了FveSnRK2.1对FveWRKY50的酸化及其对基因表达和蛋白质稳定性的影响. 分析了FveWRKY50和FveJAZ5.5之间的蛋白质-蛋白质相互作用.
主要成果:
- 异源和高内源MeJA水平都显著增加了草对毒素的敏感性.
- 在Collettotrichum感染或MeJA激活FveSnRK2.1,酸化FveWRKY50.1的情况下,确定了一个积极的反循环. 这增强了FveWRKY50的稳定性和活性,提高了JA生物合成基因 (FveAOS2,FveAOC3) 的调节,并增加了MeJA水平.
- 草蛋白FveJAZ5作为负调节剂,通过抑制FveSnRK2.1-FveWRKY50相互作用来抑制敏感性. 在病原体攻击时FveJAZ5降解释放FveWRKY50,促进易感性.
结论:
- 一个由Collettotrichum感染驱动的新的"JA信号 - JA生物合成"正反循环增强了草的易感性.
- 基因操纵,特别是淘汰FveWRKY50或过度表达FveJAZ5,使草具有显著的抵抗力.
- 这些发现为植物病原体相互作用提供了关键的见解,并为培育抗病草品种提供了宝贵的遗传资源.
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