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精子选择通过微介导的化学反应,由毛囊液和细胞引起的精子选择

Tohid Rezaei Topraggaleh¹, Amir Fattahi^2,3, Ehsan Dadkhah⁴

¹Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

|December 13, 2025

概括

毛囊液衍生的微 (FF-MVs) 增强了用于细胞内质精子注射 (ICSI) 的精子选择. FF-MVs提高了精子度,运动性和DNA完整性,为辅助生殖提供了一个新的工具.

科学领域:

生殖生物学生殖生物学
生物技术是生物技术.
辅助生殖技术辅助生殖技术

背景情况:

选择合格的精子对于成功的细胞内质精子注射 (ICSI) 是至关重要的.
基于化学反应的精子选择提供了一种有前途的方法来识别具有增强运动性和受精潜力的精子.
从毛囊液 (FF) 和积细胞 (CCs) 衍生出的微 (MVs) 被研究为潜在的化学吸引剂.

研究的目的:

通过FF-和CC-衍生的MVs诱导的化疗来评估精子选择的疗效.
评估MVs对微流体装置内的精子参数的影响.
为了确定精子丰富的FF-MVs的最佳度.

主要方法:

从接受ICSI的妇女身上收集了毛囊液 (FF) 和累积细胞 (CC).
从FF和CC中分离出微管 (MVs) 并进行了表征.
精子选择是在微流体系统中进行的,使用了来自normozoospermic男性的精子,并将MVs纳入甲板,以创建化学吸引剂梯度.
精子度,运动性,渐进性运动性和DNA完整性在选择后被分析.

主要成果:

孤立的FF和CC衍生MV表现出特有的形态和物理化学特性.
与对照组相比,在CC衍生的MV中没有观察到精子参数的显著差异.
毛囊液衍生MVs (FF-MVs),特别是50μg/mL,与对照组相比,显著改善了精子度,渐进的运动性和DNA完整性.

结论:

毛囊液衍生的微 (FF-MVs) 显示出化学吸引性质.
FF-MVs可以选择性地丰富精子种群,具有优越的功能参数.
在ICSI程序中,FF-MVs代表了增强精子选择的新有效工具.

关键词:

化学反应 (chemotaxis) 是一种积分细胞的细胞积分.毛囊液体毛囊液体微小片是微小的片.精子选择精子选择

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