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空间转录学揭示了在细胞分辨率下发展小麦花朵中的表达梯度

Katie A Long¹, Ashleigh Lister², Maximillian R W Jones¹

¹John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.

|December 13, 2025

概括

小麦的空间转录学揭示了塑造花朵发育的基因表达模式. 这项研究在细胞分辨率上绘制基因活性图,揭示了控制沿着顶-基底轴的尖状细胞形成的因素.

科学领域:

植物生物学植物生物学
发育生物学是发展生物学.
基因组学就是基因组学.

背景情况:

植物花朵结构多样性是由基因表达驱动的.
小麦尖的发育涉及延迟的基底尖形成.
之前的研究缺乏细胞分辨率的基基因表达.

研究的目的:

为了优化小麦花朵的空间转录学.
在整个发育过程中以细胞分辨率映射基因表达.
为了确定基因表达模式沿着顶 - 基底轴.

主要方法:

优化多重错误强大的光在现场混合 (MERFISH).
在4个发育阶段分析了5万个细胞.
执行细胞细分和聚类来定义表达域.

主要成果:

确定了18个不同的基因表达域和标记物.
揭示了尖和花发育的时空组织.
标志着空间协调的表达模式在树干和叶脊.

结论:

发现了对 меристем认同和过渡因素的新见解.
提供了小麦花朵发育的细胞分辨率地图.
通过交互式 WebAtlas 接口公开提供所有数据.

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