马类单细胞衍生的巨细胞重新审视:隔离和全面表征有利于与抗炎两极分化
Marguerite Meeremans1,2, Bert Devriendt3, Sarah Bairiot1
1Veterinary Stem Cell Research Unit, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium.
The veterinary quarterly
|December 13, 2025
概括
这项研究建立了马类巨分化的协议,区分了亲炎性 (IFN-γ / LPS) 和抗炎性 (IL-4) 现型. 这项研究对马的健康和作为大型动物模型具有重要价值.
科学领域:
- 兽医免疫学 兽医免疫学
- 马类生物学 马类生物学
- 细胞生物学 细胞生物学
背景情况:
- 巨细胞对于组织修复和炎症至关重要.
- 目前没有用于马类巨细胞两极分化的标准化方法.
- 马类模型在生物医学研究中越来越重要.
研究的目的:
- 开发和描述一个用于两极分化马类单细胞衍生的巨细胞的协议.
- 在马类巨细胞中定义促炎和抗炎表型.
- 评估两极化马类巨细胞的功能影响.
主要方法:
- 马类CD172a+外周血液单细胞的分离和分化.
- 使用干扰素-/脂聚糖 (IFN-γ/LPS) 或干扰素-4 (IL-4) 诱导巨分极.
- 分析形态,基因和蛋白质表达 (RT-qPCR,流细胞计,免疫光),细胞因子分泌和细胞功能.
主要成果:
- 对亲和抗炎性马类巨细胞观察到不同的形态和分子概况.
- IFN-γ/LPS刺激上调了CXCL8,CD86,IL10和TGFB1mRNA,增加了CD86蛋白和TNFα/IL-1β分泌.
- IL-4刺激上调了CXCL8,MRC1和TGFB1mRNA,增加了CD206蛋白和VEGFα的表达.
- 来自极化巨细胞的条件介质影响了肌细胞的增殖和基因表达.
结论:
- 成功开发和验证了一种用于马类巨细胞两极分化的强大协议.
- 这项研究提供了对马类巨细胞表型的全面描述.
- 这项研究对马类健康和大型动物研究具有重大物种特异性和翻译性影响.
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