皮内利亚·特纳塔-科普蒂迪斯·瑞佐马草本对通过NLRP3介导的热灭菌抑制了Helicobacter pylori的殖民化
Yukun Lin1, Kunjing Liu1, Jiahui Chen2
1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.
Journal of ethnopharmacology
|December 13, 2025
概括
皮内莉亚三角形-科普蒂迪斯瘤 (BXHL) 通过抑制粘附和激活NLRP3热灭菌来抑制Helicobacter pylori的殖民化. 这种传统中医药草药对可以保护肠道微生物群,提供安全的辅助疗法.
科学领域:
- 药理学 药理学是指药理学的学科.
- 微生物学 微生物学
- 传统中国医药 传统中国医药
背景情况:
- 皮内利亚 (Ban-xia) 和Coptidis Rhizoma (Huang-lian) 形成了一个经典的草本对 (BXHL),在临床上已证明对杆菌 (H. pylori) 的疗效.
- 需要进一步阐明BXHL抗H.pylori作用的精确药理机制.
研究的目的:
- 评估BXHL在体外和体内对抗H. pylori的殖民效应.
- 阐明其作用机制,重点关注NLRP3介导的热致死途径.
- 评估BXHL对胃肠道微生物群的影响,并确定潜在的活性成分.
主要方法:
- 使用液体染色学-并联质谱法 (LC-MS/MS) 进行了BXHL组合的特征化.
- 在GES-1细胞共同培养和感染小鼠模型中评估了抗H. pylori活性.
- 用RNA-seq,ELISA,RT-qPCR,西部斑点,IHC和16SrRNA测序来分析基因表达,信号通路和微生物群的变化.
主要成果:
- BXHL显著降低了H. pylori的粘附和殖民,恢复了由感染抑制的NLRP3介导的烧亡.
- 转录基因和分子分析证实了NLRP3信号级联的重新激活.
- 与传统的三重疗法不同,BXHL保留了肠道微生物多样性,并显示了中度的直接抗菌作用.
结论:
- BXHL通过抑制细菌粘附因子和激活NLRP3介导的热灭菌来抑制H. pylori的殖民化.
- BXHL不会破坏肠道微生物群,将其定位为安全的辅助疗法.
- 这些发现为改善H. pylori根除提供了一种新的策略,补充了传统的抗生素.
相关概念视频
Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
1.1K
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
1.1K
Acid Suppressive Drugs for Peptic Ulcer Disease: Proton Pump Inhibitors
819
Peptic ulcers, often induced by H. pylori infections or NSAID usage, arise from disruptions in the delicate balance of gastric acid production. Peptic ulcers stem from heightened gastric acid levels due to H. pylori infections or NSAID use. The protective mucus layer diminishes in the presence of these factors, allowing gastric acid to erode the stomach lining and form ulcers.
Gastric acid, a potent cocktail of hydrogen and chloride ions, is produced in specialized parietal cells within the...
Gastric acid, a potent cocktail of hydrogen and chloride ions, is produced in specialized parietal cells within the...
819
Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors
1.1K
Peptic ulcer disease, commonly called PUD, represents a multifaceted condition characterized by disruptions in the lining of the gastrointestinal (GI) tract. Central to the protection of the gastrointestinal lining is the mucosal-bicarbonate barrier. This physiological defense mechanism is a formidable shield against the corrosive effects of gastric acid and pepsin secretion in the stomach. Its role is pivotal in maintaining the structural integrity of the stomach's inner lining.
1.1K
Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists
895
Histamine H2 receptors, which are intricately located on the basolateral membrane of parietal cells, play a crucial role in modulating gastric acid secretion. When released from enterochromaffin-like cells, histamine engages H2 receptors, initiating the cyclic AMP (cAMP) pathway. In this pathway, adenylyl cyclase converts ATP into cAMP, elevating intracellular cAMP levels. The activation of protein kinase A follows, stimulating the proton pump. This stimulation prompts the secretion of hydrogen...
895
Peptic Ulcer Disease I: Introduction
711
Peptic Ulcer Disease (PUD) is characterized by mucosal excavation in the esophagus, stomach, pylorus, or duodenum. It can manifest as acute or chronic based on the extent and duration of mucosal involvement.
An acute ulcer, marked by superficial erosion and minimal inflammation, swiftly resolves upon identifying and addressing the underlying cause. In contrast, a chronic ulcer persists, potentially eroding through the muscular wall and forming fibrous tissue.
Peptic ulcers can also be...
An acute ulcer, marked by superficial erosion and minimal inflammation, swiftly resolves upon identifying and addressing the underlying cause. In contrast, a chronic ulcer persists, potentially eroding through the muscular wall and forming fibrous tissue.
Peptic ulcers can also be...
711
Peptic Ulcer Disease IV: Management
401
Medical treatment strategies for peptic ulcers encompass various methods. The primary goal of treatment is to diminish gastric acidity and strengthen mucosal defense mechanisms.
The therapeutic approach involves ensuring adequate rest, implementing drug therapy, promoting smoking cessation, making dietary modifications, and emphasizing long-term follow-up care.
Pharmacological management
The prevailing therapy for peptic ulcers involves a combination of managing the patient's current...
The therapeutic approach involves ensuring adequate rest, implementing drug therapy, promoting smoking cessation, making dietary modifications, and emphasizing long-term follow-up care.
Pharmacological management
The prevailing therapy for peptic ulcers involves a combination of managing the patient's current...
401


