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功能性苦味信号传递和刺激强度影响饮食诱导的唾液蛋白上调
Verenice Ascencio Gutierrez1, Kimberly F Beede-James2, Laura E Martin3
1Department of Psychology, State University of New York at Buffalo, Buffalo, NY, 14260, USA.
Appetite
|December 13, 2025
概括
味道信号对于唾液蛋白 (SP) 在对苦味饮食的反应中升高调节至关重要. 受到损害的味道途径阻止SP变化,即使有性刺激,突出显示味道.
科学领域:
- 生理学 生理学 生理学
- 神经科学是一个神经科学.
- 生物化学 生物化学
背景情况:
- 通过饮食接触酸和酸等苦味化合物可以增加唾液蛋白 (SP) 水平,提高饮食接受度.
- 驱动SP上调的精确机制,特别是味觉受体激活的作用,仍然不完全理解.
研究的目的:
- 调查味觉受体激活是否是对唾液蛋白的上调调节的必要组成部分,以应对苦味和缩性饮食.
- 为了确定刺激强度是否会影响唾液蛋白表达,以应对苦味化合物.
主要方法:
- 使用了缺乏功能性苦味信号通路 (TRPM5 KO和TRPM4/5 DKO) 的转基因小鼠和野生型 (WT) 小鼠.
- 在WT和KO/DKO模型中,使用含有酸 (苦和) 和酸 (苦) 的不同度的饮食.
- 分析了唾液蛋白表达模式,以应对不同的饮食刺激和度.
主要成果:
- TRPM5 KO和TRPM4/5 DKO小鼠在食酸饮食时未能上调SPs,尽管能够通过异二醇注射做到这一点.
- 作为对酸饮食的反应,WT小鼠成功地提高了SPs,而KO模型没有,这表明需要完整的味道信号.
- 在大鼠中,因暴露导致了特定14kDa频段的度依赖的SP上调,而糖乙酸盐 (SOA) 则没有影响蛋白质表达.
结论:
- 味道信号通路对于唾液蛋白在对苦味和缩性饮食成分的反应中进行上调至关重要.
- 仅仅是严谨性,没有苦味激活,就不足以触发唾液蛋白质的变化.
- 苦刺激的强度可以调节唾液蛋白上调的程度,以度依赖的方式.
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