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一个手动驱动的离心微流体LAMP平台,用于在水上运动中快速视觉检测水传播病原体

YanJing Chen¹, LinXiao Wu¹, WangXin Ding¹

¹Capital University of Physical Education and Sports, Beijing, 100191, China.

Scientific reports

|December 13, 2025

概括

这项研究开发了一种集成的护理点测试 (POCT) 平台,用于快速,可视检测水传播病原体,如金黄色葡萄球菌和柔性菌,为水生安全提供了用户友好的解决方案.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
公共卫生公共卫生

背景情况:

水上运动由于水中传递的病原体而造成公共卫生风险.
传统的病原体检测方法缓慢,依赖实验室,不适合快速现场检测.
迫切需要有效的临床检测解决方案.

研究的目的:

开发和验证一个集成的,用户友好的POCT平台,用于检测多种水传播病原体.
创建一个快速,具有成本效益的系统,用于水生环境的现场病原体检测.
在POCT系统设计中展示"后端补偿"的概念.

主要方法:

开发了一个由三个模块组成的平台:无仪器核酸提取,用于样品分配的PMMA微流体芯片,以及基于视觉pH指标的循环介导同热放大 (LAMP) 试验.
优化了用于检测金黄色葡萄球菌和Shigella flexneri的原料和化学成分.
与常规方法和模拟污染水相比,经过验证的平台性能.

主要成果:

在~60分钟内实现了样本到答案的工作流程,检测限制为10-100个副本/反应.
微流体芯片与基于管的测试相比,显示出更高的灵敏度和稳定性,特别是对于低滴度样本.
简化提取显示了与商业套件相比的效率,用于克负细菌,以及对干扰物的高特异性.

结论:

集成的POCT平台为现场水产安全监测提供了可行的,具有成本效益的解决方案.
该平台的设计验证了"后端补偿",其中强大的检测抵消了简化样本准备.
这种方法为为资源有限的环境开发可靠的POCT系统提供了宝贵的见解.

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