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循环RNA PTPN4 在早期发生过程中导致血脑屏障破坏

Jiurong Yang¹, Yang Hu¹, Feiyu Wang¹

¹Department of Pharmacology, Medical School of Southeast University, Nanjing, 210009, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|December 14, 2025

概括

循环RNA PTPN4 (CircPTPN4) 在早期中升高,破坏了血脑屏障. 准这种循环RNA可能会保持屏障完整性并减少发作.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

循环RNAs (circRNAs) 调节基因表达,并与大脑发育和病理学有关.
涉到复杂的分子变化,影响大脑功能和完整性.

研究的目的:

调查CircPTPN4在发生和血脑屏障 (BBB) 完整性中的作用.
阐明CircPTPN4在中的功能背后的分子机制.

主要方法:

小鼠叶发作模型.
对脑组织和血中CircPTPN4表达的分析.
研究CircPTPN4对大脑微血管内皮细胞 (BMEC) 和BBB完整性的影响.
涉及RNA测序,miRNA分析和途径分析的分子机制研究 (p38/MAPK).

主要成果:

在早期的发发生过程中,CircPTPN4的表达升高.
电路PTPN4过度表达会破坏BMEC的紧密结,损害BBB的完整性.
电路PTPN4作为miR-145a-5p的分子海绵,上调ECE-1和随后ET-1的产生.
通过ET-1激活p38/MAPK通路,可以降低紧结蛋白的调节.
Knockdown 的 CircPTPN4 改善了 BBB 干扰,并减少了发作频率.

结论:

环PTPN4/miR-145a-5p/ECE-1轴是早期发症和BBB功能障碍的关键参与者.
CircPTPN4作为早期的潜在诊断生物标志物.
向CircPTPN4提供了一种治疗策略,用于维护BBB完整性和治疗.

关键词:

血脑屏障是什么意思环PTPN4 环PTPN4 环输内甲烯转化酶-1-1的转化酶.是一种.在 miR-1455 的情况下.

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