铁驱动的微生物协同作用增强了在硫化物应激下减少脱的作用
Che-Wei Lu1, Po-Sheng Kuo2, Ku-Fan Chen3
1Department of Life Sciences, National Central University, Taoyuan 32001, Taiwan; Research and Development Department, Overchlorine Corporation, Taoyuan 32001, Taiwan.
Journal of hazardous materials
|December 14, 2025
概括
硫化物毒性抑制了三乙烯 (TCE) 的脱. 铁 (Fe(II)) 的添加沉硫化物,通过重新激活关键细菌来恢复TCE降解,并增强地下水修复的微生物伙伴关系.
科学领域:
- 环境微生物学 环境微生物学
- 生物修复是一种生物修复.
- 地质化学 地质化学
背景情况:
- 乙烯 (CE) 和三乙烯 (TCE) 污染了地下水,通常含有高硫酸盐水平.
- 减少硫酸盐的细菌产生硫化物,严重抑制Dehalococcoides mccartyi (Dhc),对TCE分解至关重要.
- 硫化物对Dhc的毒性是显著的,几乎消除了超过5毫米度的TCE脱.
研究的目的:
- 制定一项战略,以克服共同污染的含水层中硫化物抑制TCE脱的方法.
- 为了研究一个协同系统的有效性,涉及DHC和硫酸盐降解细菌与铁化物补充.
主要方法:
- 与Dhc菌株CWV2和Nitratidesulfovibrio liaohensis菌株KPS的共同栽培.
- 补充铁化物 (Fe(II)) 以沉硫化物作为铁硫化物 (FeS).
- 转录组分析以评估 Dhc 和 KPS 中的基因表达变化.
- 微生物群体分析,以评估细菌群体和功能伙伴的变化.
主要成果:
- 铁补充剂有效地通过形成FeS来排毒硫化物,恢复DHC介导的TCE脱.
- 确定了最佳的Fe/S摩尔比率,以最大限度地去除硫化物,而不会产生不良的pH或矿物质形成.
- FeS治疗重新激活了 Dhc 的脱酶和酶基因,并增强了 KPS 的硫代谢.
- 微生物群落转移,增加了DHC的丰富性,并丰富了产生的发酵细菌.
结论:
- 以Fe (II) 为媒介的硫化物沉是一种可行的策略,用于TCE硫酸盐共同污染的地下水的现场整治.
- 这种方法稳定了微生物相互作用,维持了必要的减少脱过程.
- 协同效应系统为复杂的地下水污染挑战提供了切实可行的解决方案.
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