评价kombucha的芳香分析的色谱技术
Sarah C Foster1, Laura Tipton2, Katelynn A Perrault Uptmor3
1Nontargeted Separations Laboratory, Chemistry Department, William & Mary, 540 Landrum Drive, Williamsburg, VA, 23185, USA.
综合的二维气相色谱 (GC×GC-TOFMS) 提供了优越的分析kombucha.
科学领域:
- 食品化学 食品化学
- 分析化学 分析化学
- 微生物学 微生物学
背景情况:
- 康布查,一种发酵茶,具有复杂的芳香特征,尚未得到充分理解.
- 挥发性有机化合物 (VOCs) 在发酵食品的风味和香味方面发挥着重要作用.
- 需要先进的分析技术来充分描述kombucha的化学成分.
研究的目的:
- 为了比较单维气体染色学-飞行质谱学时间 (GC-TOFMS) 和综合二维气体染色学-飞行质谱学时间 (GC×GC-TOFMS) 的分析有效性.
- 用它们的VOC档案来区分各种kombucha产品.
- 阐明kombucha中的VOCs的起源,区分茶叶衍生和微生物发酵衍生化合物.
主要方法:
- 使用GC-TOFMS和GC×GC-TOFMS分析了12个kombucha样本 (包括SCOBY,初级茶和商业/本地kombuchas).
- 应用统计方法,如主要组件分析 (PCA),主要坐标分析 (PCoA),层次集群分析 (HCA),费舍尔比率,并对VOC数据的折叠变化.
- 识别和量化挥发性有机物,以建立化学概况.
主要成果:
- 与GC-TOFMS相比,GC×GC-TOFMS检测到更多的分析物 (182) 和化合物类 (17),比GC-TOFMS (127个分析物,15个类).
- GC×GC-TOFMS成功地解决了GC-TOFMS数据中的coelutions,提供了更高的分辨率.
- 观察到不同的VOC档案:本地生产的kombucha显示了简单的和酸,而商业品牌则显示了烯和芳香化合物.
结论:
- GC×GC-TOFMS是一种比GC-TOFMS更强大的技术,用于详细描述康布恰的挥发性芳香特征.
- 挥发性有机化合物分析可以有效地区分不同类型的kombucha产品.
- 这项研究提供了有关将微生物和茶叶衍生的VOC与发酵饮料的整体风味特征的见解.
更多相关视频
08:43PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
Published on: May 11, 2017
05:29Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
相关概念视频
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Chromatographic Methods: Terminology
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
Principles Of Column Chromatography
