由CRISPR/Cas9工程设计的Bacillus subtilis底盘,用于从海洋废弃物中量身定制的基托醇糖生产
Jiayu Liang1,2,3, Tengyue Liang1,2,3, Chengjia Wei1,2,3
1University Engineering Research Center of Advanced Technologies in Medical and Biological Intelligent Manufacturing, Guangxi Medical University, Nanning, 530021, China.
Microbial cell factories
|December 14, 2025
概括
这项研究开发了一种可持续的生物工艺,使用工程 Bacillus subtilis 来将海洋废物转化为有价值的基托醇糖 (COS). 工程细菌高效地产生胆酶,为传统的化学方法提供了一个环保的替代方案.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 海洋生物技术 海洋生物技术
背景情况:
- 传统的生产基托醇糖 (COSs) 的方法往往会产生化学废物.
- 海洋废物中含有丰富的酸盐,是未充分利用的资源.
- 需要可持续和环保的生物工艺来生产高价值化合物.
研究的目的:
- 建立一个可持续的生物工艺,将海洋衍生的酸盐转化为有价值的酸 (COSs).
- 为了设计Bacillus subtilis作为一个细胞工厂,用于异质基托酶的生产.
- 为了优化发酵过程,实现高产量的COS生产.
主要方法:
- 通过使用CRISPR/Cas9.9删除其内源性胆酶基因 (BsCsn) 来改造Bacillus subtilis WB800N.
- 表达了来自Streptomyces coelicolor的编码子优化的GH46胆酶 (CsnA),与AprE信号融合.
- 在DO-stat控制的料批次条件下,在5L生物反应器中使用响应表面方法优化发酵过程.
主要成果:
- 获得了540.08±6.20U/mL的高细胞外CsnA活性.
- 获得了11.25U/mL·h的生产率和1682.86U/g的碳转化效率.
- 通过MALDI-TOF MS分析确认了具有定义的聚合度 (DP2-DP4) 的COSs的生产.
结论:
- 建立了一个集成的平台,将海洋废物回收回收成高价值的COS.
- 证明了Bacillus subtilis作为一个生态高效的细胞工厂用于基托桑酶的生产.
- 提供了一个有价值的框架,用于Bacillus subtilis中其他基因酶的异质表达.
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