Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Modified-Release Drug Delivery Systems: Rate-Programmed II01:19

Modified-Release Drug Delivery Systems: Rate-Programmed II

137
Rate-programmed drug delivery systems release drugs in a controlled manner to maintain therapeutic levels. Three main designs include reservoir, matrix, and hybrid systems.Reservoir systems consist of a drug core enclosed within a membrane that controls drug release. In non-swelling reservoir systems, polymers like ethyl cellulose or polymethacrylates are used. These do not hydrate in aqueous media and control release through membrane thickness, porosity, or insolubility. This type includes...
137
Modified-Release Drug Delivery Systems: Rate-Programmed I01:22

Modified-Release Drug Delivery Systems: Rate-Programmed I

172
Rate-programmed drug delivery systems (DDS) are designed to release drugs at specific, controlled rates to maintain consistent therapeutic levels. These systems are categorized based on their release mechanisms, including dissolution-controlled DDS, diffusion-controlled DDS, and combined dissolution-diffusion-controlled DDS.In dissolution-controlled DDS, the release rate depends on the slow dissolution of the drug itself or the surrounding matrix. Drugs with inherently slow dissolution rates,...
172
Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

164
Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
164
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

160
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
160

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Multi-Property Optimization of Antimicrobial Peptides Using Reinforcement Learning and Conditional Independence Regularization.

IEEE journal of biomedical and health informatics·2026
Same author

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same author

Mitigating Electrode Stress via Self-Constructed Interfacial Carrier Networks in High-Areal-Capacity SiO<sub><i>x</i></sub> Anodes.

Journal of the American Chemical Society·2026
Same author

Trans-dimerization of Amyloid Precursor Protein family members induces pre- and postsynaptic differentiation through distinct signaling pathways.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Schizophrenia-associated glycoprotein Adamtsl3 regulates perineuronal net formation, maintenance, and adult cortical plasticity.

Molecular psychiatry·2026
Same author

Comparative reproductive toxicity of 6:2 FTS and PFOS in female zebrafish (Danio rerio): From histopathology to molecular response.

Ecotoxicology and environmental safety·2026

相关实验视频

Updated: May 5, 2026

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

13.8K

通过精确编程ARRDC1-介导的微小小管的向细胞内传递.

Zhi Qiao1, Sengjin Choi1, Zunwei Chen1

  • 1Departments of Environmental Health & Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA.

Journal of extracellular vesicles
|December 15, 2025
PubMed
概括

研究人员开发了工程微微粒 (ARMMs) 来针对性地传递mRNA和CRISPR基因编辑器. 这种平台能够精确地将治疗性宏分子传递给特定的细胞类型,从而推进基因和RNA基因医学.

关键词:
在 ARMM 中,有 ARMM.对于mRNA和CRISPR疗法的使用.非病毒传递的非病毒传递.精准医学是一门精准医学.有针对性的交付目标.

更多相关视频

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

13.3K
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
10:16

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Published on: February 8, 2017

8.0K

相关实验视频

Last Updated: May 5, 2026

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

13.8K
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

13.3K
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
10:16

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Published on: February 8, 2017

8.0K

科学领域:

  • 生物技术是生物技术.
  • 分子医学是分子医学.
  • 细胞生物学 细胞生物学

背景情况:

  • 高效和细胞特异性输送核酸和基因编辑工具,如mRNA和CRISPR对于治疗应用至关重要.
  • 目前的输送方法在向特定细胞群体时面临挑战,限制治疗疗效.

研究的目的:

  • 开发一个多功能和可编程的输送平台,用于针对性地输送治疗性大分子.
  • 通过细胞特异性连接物,设计微微囊,选择性地向定义的细胞类型输送货物.

主要方法:

  • 工程ARRDC1-介导的微 (ARMMs) 被尼帕病毒 (NiV) 衍生的蛋白质和细胞特异性配体 (针对GluA4的抗CD8 scFv或DARPin) 装饰.
  • ARMMs被功能化以准CD8+ T细胞和帕瓦胺阳性 (PV+) 神经元.
  • 进行了体外和体外研究,以评估目标交付效率和功能性货物释放.

主要成果:

  • 与抗CD8 scFv功能化的ARMM选择性地将蛋白质,mRNA和CRISPR-Cas9基编辑器传递给CD8+ T细胞.
  • 显示针对GluA4的DARPin的ARMMs使得向PV+神经元的传递成为可能.
  • 在体内给药证明了对小鼠的CD8+脊髓细胞和PV+皮层神经元的功能传递.

结论:

  • 表面工程ARMM代表了一个可编程和模块化系统,用于精确地传递治疗性宏分子.
  • 这个平台在基因和RNA医学中具有广泛的应用性,克服了关键的交付障碍.
  • 这项研究建立了一种针对mRNA和基于CRISPR的治疗方法的新方法.