双模式多通道成像系统用于高通量活细胞监测,跨大视野
Christos Katopodis1, Dimitris G Papazoglou2,3, Ioanna Zergioti1
1National Technical University of Athens, School of Applied Mathematical and Physical Sciences, Athens, Greece.
Journal of biomedical optics
|December 15, 2025
概括
我们为器官在芯片 (OoaC) 平台开发了一种新的双模式成像系统,克服了对高分辨率活细胞成像的放大视野权衡. 这种可扩展的解决方案增强了微流体生物分析和药物查.
科学领域:
- 生物光子学和成像系统
- 微流体学和芯片上的实验室技术
- 细胞和分子生物学 细胞和分子生物学
背景情况:
- 高通量活细胞成像对于生物研究至关重要,特别是对于器官在芯片 (OoaC) 平台.
- 传统的光学系统在平衡放大与视野 (FOV) 中面临局限性,这阻碍了在单细胞分辨率下捕捉大规模的生物动态.
研究的目的:
- 为微流体设备开发一种双模式,多通道的光学成像系统.
- 该系统旨在在扩展的FOV中实现单细胞分辨率,同时保持合适的工作距离,以便与微流体系统集成.
主要方法:
- 利用微镜头阵列和激光制造的微孔阵列来光学隔离成像通道并最大限度地减少交叉声.
- 实现了两个操作模式:快速采样即时成像和全场成像与微扫描和计算拼接用于高分辨率复合材料生成.
主要成果:
- 在单细胞分辨率和14毫米工作距离下,在4倍放大时实现了8.4 × 6毫米2的宽FOV.
- 实验结果验证了理论模型,证明了不需要大型传感器的高保真成像.
- 双模式功能允许快速评估和详细,大面积成像.
结论:
- 开发的紧且可扩展的成像系统有效地克服了传统的放大-FOV权衡.
- 这项技术为药物查,细胞动态研究和基于微流体的生物分析提供了强大的工具.
- 该系统的适应性和高分辨率能力对于推进芯片器官技术非常有价值.
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