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相关概念视频

Nucleic Acid Structure01:25

Nucleic Acid Structure

8.3K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.3K
RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K
RNA Structure01:23

RNA Structure

78.7K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.7K
RNA Structure01:19

RNA Structure

6.9K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.9K

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相关实验视频

Updated: Jan 8, 2026

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

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RNA-EFM:基于蛋白质条件的RNA序列结构共设计的基于能源的流量匹配.

Abrar Rahman Abir1, Liqing Zhang2,3

  • 1Department of Computer Science and Engineering, Bangladesh University of Engineering and Technology, Dhaka 1000, Bangladesh.

Bioinformatics advances
|December 15, 2025
PubMed
概括

我们开发了RNA-EFM,这是一个深度学习框架,用于设计结合蛋白质的RNA分子. 它通过整合生物物理因素来提高RNA稳定性和结合精度,优于现有方法.

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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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相关实验视频

Last Updated: Jan 8, 2026

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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
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科学领域:

  • 计算生物学是一种计算生物学.
  • 生物物理学的生物物理.
  • 机器学习用于RNA设计.

背景情况:

  • 设计用于特定蛋白质结合的RNA分子对于生物和治疗应用至关重要.
  • 当前的方法往往忽略了关键的生物物理特性,如稳定性和热力学可行性.

研究的目的:

  • 引入RNA-EFM,这是一种用于蛋白质条件RNA序列和结构共设计的新型深度学习框架.
  • 通过结合生物物理约束来解决现有的RNA设计方法的局限性.

主要方法:

  • RNA-EFM利用流量匹配来对RNA骨干结构进行几何对齐.
  • 一个基于能量的精细化组件通过物理能量和生物物理先验 (列纳德-斯潜力,自由能量) 代地增强RNA结构预测.

主要成果:

  • RNA-EFM显著提高了RNA设计精度,在RMSD,lDDT,序列恢复和结合能量方面表现优于最先进的基线.
  • 该框架产生了几何可信和热力学稳定的RNA分子.

结论:

  • 纳入生物物理约束对于有效的RNA设计至关重要.
  • RNA-EFM代表了用于各种应用的计算RNA设计的有希望的进步.