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衰老对天体细胞对大脑内皮细胞信号传递的影响

Shuzhen Guo¹, Gen Hamanaka¹, Fang Zhang¹

¹Neuroprotection Research Laboratory, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School Charlestown MA USA.

Neuroprotection (Chichester, England)

|December 15, 2025

概括

星球细胞中的细胞衰老通过增加血管素原 (AGT) 信号传递,损害了血脑屏障 (BBB). 降低老年星球细胞中的AGT恢复了BBB功能,这表明对老年中枢神经系统疾病的治疗点.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
衰老研究研究衰老研究

背景情况:

衰老显著影响中枢神经系统 (CNS) 功能,包括血脑屏障 (BBB) 的完整性.
细胞衰老,衰老的标志,正在通过星球细胞-大脑内皮细胞相互作用对改变BBB功能的潜在作用进行研究.

研究的目的:

为了确定星细胞衰老是否影响BBB属性.
调查血管素信号传递在调解与天体细胞衰老相关的BBB变化的作用.

主要方法:

人类天体细胞和大脑微血管内皮细胞在低通道和高通道培养,以诱导衰老.
通过β-galactosidase染色和CDKN2A基因表达来确认衰老.
共同培养被用来评估大脑内皮细胞的透性,使用Alexa Fluor 488标记的BSA追踪器.
在衰老的星球细胞中使用siRNA调节 angiotensinogen (AGT) 表达.

主要成果:

年轻的天体细胞增强了BBB基因表达,降低了内皮透性.
衰老的星体细胞失去了促进BBB完整性的能力 (122.8%±28.6%对比45.5%±18.0%).
在衰老的星体细胞中,AGT水平升高,通过siRNA减少AGT恢复了老年星体细胞的BBB促进作用.

结论:

星球细胞增加的血管激素信号,有助于降低衰老中枢神经系统中的BBB功能.
向星性血管激素信号可能提供一个策略,以减轻与年龄相关的BBB功能障碍.

关键词:

衰老的衰老衰老的衰老血管新生素是一种星球细胞是星球细胞.血脑屏障血脑屏障血脑屏障血脑屏障细胞内皮的内皮.衰老是一种老化.

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