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Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

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Updated: Jun 5, 2026

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Published on: October 13, 2021

生物反应器启用细胞外囊生产,用于下游功能工程.

Thaddeus L Tripp, Karina Vasile, Massimo Terrizzi

bioRxiv : the preprint server for biology

|December 15, 2025

概括

高密度生物反应器显著增加了细胞外囊泡 (EV) 的产生,并使治疗应用的高效工程成为可能. 这些可扩展的系统克服了传统细胞培养的局限性,推进了基于EV的药物输送.

科学领域:

生物技术是生物技术.
细胞生物学细胞生物学
纳米医学是一种纳米医学.

背景情况:

细胞外囊泡 (EVs) 显示出治疗前景,但由于生产产量低和工程效率低,在临床转化方面面临挑战.
目前使用标准瓶培养的方法限制了EVs的可扩展性和下游修改.
对于强大的电动汽车生产和多功能后隔离工程,需要高密度生物反应器系统.

研究的目的:

系统地比较不同培养系统的EV生产和工程能力:瓶式,膜式生物反应器和空心纤维生物反应器.
评估生物反应器衍生电动汽车的可扩展性,资源效率和翻译可行性.
通过后期生产工程来证明生物反应器生产的EVs在先进的药物输送应用中的潜力.

主要方法:

使用纳米粒子跟踪分析,免疫染和传输电子显微镜对EV生产进行比较分析.
蛋白质组分析以确认正规的EV表型并评估翻译潜力.
新型电动汽车工程技术的演示:通过电动汽车微粒混合化进行货物装载,并通过微粒携带的分子后插入进行表面修饰.

主要成果:

与瓶培养相比,空洞纤维生物反应器的EV度显著更高,中介消耗减少.
生物反应器衍生的EV保持了基本的表型,并证明了治疗修改的高工程性.

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Published on: August 16, 2024

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Last Updated: Jun 5, 2026

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Published on: October 13, 2021

Author Spotlight: The Production of Recombinant Proteins

Author Spotlight: The Production of Recombinant Proteins

Published on: June 30, 2023

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Published on: August 16, 2024

新型电动汽车工程方法成功应用于生物反应器生产的电动汽车,只需要最小的电动汽车数量.

结论:

生物反应器平台,特别是空心纤维和基于膜的系统,克服了传统电动汽车生产方法的吞吐量限制.
这些可扩展的生物反应器可以生产适合先进药物输送应用的可设计电动汽车.
该研究确立了生物反应器作为面向翻译的系统,用于增强EV生产和治疗开发.