相关实验视频

Updated: Jan 8, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.3K

使用CRISPore-seqq对替代拼接调节者的全转录组分析

Simon Müller, Nathanael Andrews, Rachel E Yan

    bioRxiv : the preprint server for biology
    |December 15, 2025
    PubMed
    概括

    通过将CRISPR扰动与长读测序相结合,CRISPore-seq使单细胞对替代拼接的分析成为可能. 这种方法揭示了成千上万的剪接变化及其对基因功能的影响,推动了转录学研究.

    更多相关视频

    Using the E1A Minigene Tool to Study mRNA Splicing Changes10:25

    Using the E1A Minigene Tool to Study mRNA Splicing Changes

    Published on: April 22, 2021

    5.4K
    Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay11:22

    Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

    Published on: August 26, 2018

    9.2K

    相关概念视频

    Alternative RNA Splicing02:18

    Alternative RNA Splicing

    Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
    There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
    24.6K
    Alternative RNA Splicing02:18

    Alternative RNA Splicing

    4.7K
    CRISPR and crRNAs02:53

    CRISPR and crRNAs

    Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
    The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
    18.7K
    Ribosome Profiling02:24

    Ribosome Profiling

    Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
    Applications of ribosome profiling
    Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
    The technique...
    4.0K
    CRISPR01:59

    CRISPR

    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
    57.4K
    Chromatin Structure Regulates pre-mRNA Processing02:41

    Chromatin Structure Regulates pre-mRNA Processing

    In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
    The chromatin structure, especially...
    8.1K