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阐明AML核糖体生物发生,重塑预后评估和免疫微环境的综合单细胞和散装RNA分析
Xiaohe Chen1, Aimei Feng2, Fan Chen1
1Department of Blood Transfusion, The Third Affiliated Hospital of Wenzhou Medical University, Rui'an, China.
Frontiers in immunology
|December 15, 2025
概括
这项研究表明,核糖体生物发生活动在急性髓性白血病 (AML) 中至关重要. 一个新的预后签名预测患者的结果和免疫疗法反应,为改善AML治疗提供了洞察力.
科学领域:
- 血液学 血液学 血液学
- 分子生物学分子生物学
- 计算生物学 计算生物学
背景情况:
- 由于其异质性和高复发率,急性髓性白血病 (AML) 存在重大挑战.
- 不调节的核糖体生物发生与癌症的发展和治疗耐药性有关.
- 核糖体生物发生在AML预后和免疫微环境中的精确作用需要进一步研究.
研究的目的:
- 构建AML的单细胞地图,并量化核糖体生物发生活动.
- 开发和验证基于核糖体生物发生的AML可解释的预后签名.
- 评估这种特征与临床结果,免疫微环境和治疗反应的关联.
主要方法:
- 单细胞RNA测序和计算分析,以创建一个AML地图.
- 机器学习 (随机生存森林) 开发一个预后性核糖体生物发生签名 (RBS).
- 通过分子技术进行模型解释性和验证的SHapley添加式解释 (SHAP).
主要成果:
- 在AML干细胞和原始细胞中观察到高的核糖体生物发生活性评分 (RAS).
- 开发的RBS表现出强大的预测能力,EXOSC2被确定为关键预测因子.
- 低RBS患者表现出免疫激活的微环境和更好的免疫治疗反应.
结论:
- 核糖体生物发生在AML进展中起着至关重要的作用.
- 可解释的RBS签名可以评估免疫治疗的响应性,并识别新的治疗点.
- 这种预后工具为临床决策提供了宝贵的见解,以提高AML患者的治疗结果.
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