隔离和描述用于控制细菌感染的沙门氏菌菌体
Seyyed Danial Mirmiran1,2,3, Xinxin Li1,2,3, Xiangmin Li1,2,3,4
1National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China.
Frontiers in veterinary science
|December 15, 2025
概括
耐多药沙门氏菌 (Salmonella enterica) 是一个主要威胁. 菌体DN01,DN03,DN19和DN28表现出强烈的化活性,是安全,稳定,并有效控制小鼠的沙门氏菌感染.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 兽医医学 兽医医学 兽医医学
背景情况:
- 多药耐药 (MDR) 沙门氏菌 (Salmonella enterica) 构成了严重的公共卫生和食品安全挑战.
- 菌体被探索为抗生素的有希望的替代品,因为它们的特异性和无法破坏共生微生物群.
研究的目的:
- 为潜在的治疗应用孤立和描述沙门氏菌特异性菌菌体.
- 评估针对MDR沙门氏菌株和小鼠感染模型中选定的菌体的疗效.
主要方法:
- 在中国,从各种环境来源中分离出沙门氏菌种特异性菌体.
- 四种菌体 (DN01,DN03,DN19,DN28) 的表征,包括形态学 (TEM),基因组测序和稳定性评估 (pH,温度).
- 在实验室中对沙门氏菌感染小鼠模型中的抗生物膜活性和体内疗效的评估.
主要成果:
- 菌体对多种沙门氏菌血清病毒,包括MDR菌株,表现出强烈的催化活性,具有广泛的宿主范围和在广泛的pH和温度范围内稳定.
- 基因组分析证实了 lysogeny,毒性或抗生素耐药性基因的缺失,将菌体归类为Caudoviricetes类.
- 菌体治疗显著抑制了细菌生长,破坏了生物膜,并显著提高了受感染小鼠的生存率,菌体尾酒实现了100%的生存率.
结论:
- 描述的细菌菌体 (DN01,DN03,DN19,DN28) 是安全,稳定和有效的对抗MDR Salmonella enterica的药物.
- 菌体治疗,特别是使用尾酒配方,在公共卫生和兽医环境中对控制沙门氏菌感染具有重大前景.
相关概念视频
01:30Lytic Cycle of Bacteriophages
77.3K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
77.3K
01:26DNA Bacteriophages
744
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
744
00:43Lysogenic Cycle of Bacteriophages
67.2K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
67.2K
01:28Biological Methods for Microbial Control
758
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
758
01:28Gene Regulation in Microbial Communities: Quorum Sensing
463
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
463
01:16Viral Replication: Lysogenic Cycle
1.2K
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
1.2K