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中性细胞外细胞线粒体具有对黄金葡萄球菌的直接细菌静止/杀菌活性

Miguel Angel Rivero-Silva¹, María Maximina Bertha Moreno-Altamirano¹, Ricardo Mondragón-Flores²

¹Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de Mexico, Mexico.

|December 15, 2025

概括

来自中性粒细胞的细胞外线粒体可以抑制细菌的生长,诱导Staphylococcus aureus的可活但不可培养的状态. 这种抗微生物效应部分由线粒体的活性氧物种介导.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
细胞生物学细胞生物学

背景情况:

中性粒细胞是关键的免疫细胞,通过各种机制对抗感染.
中性粒细胞释放的细胞外线粒体可以为细菌标记增强的细胞化.
来自细菌的线粒体可能具有内在的抗微生物特性.

研究的目的:

研究细胞外线粒体的直接细菌静止和杀菌潜力.
为了确定中性粒细胞的线粒体对金黄色葡萄球菌的生长和生存能力的影响.
探索线粒体反应性氧物种 (mROS) 在这种抗菌活性中的作用.

主要方法:

隔离了中性粒细胞的线粒体,并将它们与金黄色葡萄球菌共同培养.
使用殖民地形成单位 (CFU) 和光学密度评估细菌生长.
使用LIVE/DEAD测试和电子显微镜分析细菌活力和线粒体-细菌相互作用.
测试了线粒体反应性氧物种 (mROS) 抑制剂MitoQ的作用.

主要成果:

细胞外线粒体显著减少了细菌的生长,但并没有杀死大多数细菌.
大多数黄金葡萄球菌的培养都表现出一种可生存但不能培养 (VBNC) 的状态.
电子显微镜揭示了细菌和线粒体的形态变化,包括某些细菌的细胞壁破坏.
MitoQ部分逆转了生长抑制,表明mROS参与,但没有影响细菌活力或相互作用.

结论:

没有细胞的细胞外线粒体表现出直接的细菌静止和一些杀菌活性对金黄色葡萄球菌.
这种抗微生物效应部分由线粒体的活性氧物种 (mROS) 介导.
细胞外线粒体可以诱导细菌中的VBNC状态,影响细菌的生存策略.

关键词:

这里是mROS.细菌细菌细菌是一种细菌.具有细菌静止/杀菌活性.生物体内共生 (endosymbiosis) 是一种生物体内共生.线粒体中的线粒体.

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