相关实验视频

Updated: Jan 8, 2026

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Published on: January 18, 2014

在Bacillus amyloliquefaciens中单个和多个基因的调节,通过体内进化系统进行调节

Cong Jiang¹, Yanqiu Liu¹, Wenyuan Han¹

¹National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan 430070, China.

ACS synthetic biology

|December 15, 2025

概括

这项研究在Bacillus amyloliquefaciens中开发了一种有效的体内进化系统,用于基因调节. 该系统成功地提高了工程菌株中生产的93%以上.

科学领域:

合成生物学合成生物学
代谢工程是代谢工程.
分子生物学分子生物学

背景情况:

合成生物学通过体内进化系统加速了细胞工厂的建设.
氨基面菌是一种关键的工业微生物.

研究的目的:

开发一种高效的体内进化系统,用于调节Bacillus amyloliquefaciens中的单个和多个基因.
为了增强工程化Bacillus amyloliquefaciens菌株的血红素生产.

主要方法:

构建了一个CRISPR/Cas9n-AID基数编辑器,用于高效的C-T基数转换 (高达90%).
设计了一个进化模板,用于体内进化的可编辑RBS序列.
利用下一代测序来确定进化的最佳扩展 sgRNA 策略.
采用性蛋白酶基因 (aprE) 进行单基因进化,并同时进化多个血红蛋白生物合成基因 (dhemA, SAM2, hemEHY).

主要成果:

通过单基因进化在突变菌株中实现了渐变AprE活动.
开发出一种最佳突变菌株 (HZHA-C2),产生14.02毫克/升血,与对照组相比增加了93%.
由于hemH基因的过度表达,血红素标量增加了49%.
在基因工程菌株 (HZHA2/pHY-hemH) 中,通过食批发发酵进一步提高了血红素标位,达到32.61 mg/L (64%的增加).

结论:

开发的体内进化系统是有效的单个和多个基因调节在Bacillus amyloliquefaciens.
改造后的菌株显示出明显增强的血红素生产能力.
这个系统提供了一个强大的工具,可以加速细胞工厂的发展,以生产有价值的化合物.

关键词:

亚米洛基面菌 (Bacillus amyloliquefaciens) 是一种有毒的植物.基础编辑器基础编辑器这里是他的家园.在生物体内进化的进化.代谢网络是代谢网络.

更多相关视频

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Published on: November 24, 2017

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

相关概念视频

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Global Regulatory Systems

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

Coordination of Gene Expression Processes in Bacteria

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...