评估来自单细胞轨迹的马赛克和监管发展模式的相对贡献
Solène Song1, Paul Villoutreix1
1Aix-Marseille Université, MMG, INSERM U1251, Turing Centre for Living Systems, Marseille, France.
PLoS computational biology
|December 15, 2025
概括
这项研究使用C. elegans.单细胞数据量化了马赛克和调节性发育模式. 它揭示了这些模式在组织和发育阶段的不同贡献,为发育生物学提供了一种新的方法.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 系统生物学 系统生物学
背景情况:
- 在发育过程中,细胞命运的决定通常遵循马赛克或调节模式.
- 在单细胞分辨率上量化马赛克和调节模式的相对贡献仍然具有挑战性.
研究的目的:
- 开发和应用一种新的定量方法来衡量马赛克和监管发展模式的贡献.
- 用单细胞蛋白质表达数据分析C. elegans发育中的细胞命运决定.
主要方法:
- 利用来自C. elegans发展的不变血统和空间数据.
- 定义了两个指标来量化马赛克 (血统表达关系) 和调节 (上下文表达关系) 模式.
- 使用人工发育模型验证的指标.
主要成果:
- 证明了C. elegans发育中的马赛克和调节模式的共存.
- 表明这些模式的相对贡献在不同的组织和发育阶段有所不同.
- 随着时间的推移,观察到皮肤组织从马赛克转变为调节性主导地位.
结论:
- 开发的方法提供了一种定量,公正和无干扰的方法来研究发育原则.
- 这项工作为细胞命运决定的基本机制提供了新的见解.
- 这些发现突出了发展中的内在 (马赛克) 和外在 (监管) 因素之间的动态相互作用.
相关概念视频
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...