植物蛋白-黄素纳米颗粒:蛋白质特征如何塑造粒子特性
Xiang Lin1, Xiaoting Zhai2, Wenting Huang1
1The Key Laboratory of Novel Enzyme Design and Creation of Fujian Province, College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, Fujian, PR China.
International journal of biological macromolecules
|December 15, 2025
概括
植物蛋白质为黄素创造稳定的纳米颗粒,改善其传递. 蛋白质结构影响纳米粒子组装,并在消化过程中保护黄素,提高生物可用性.
科学领域:
- 食品科学与技术 食品科学与技术
- 材料科学 材料科学 材料科学
- 生物化学 生物化学
背景情况:
- 黄素 (Cur) 是一种疏水性营养药,具有较差的水溶性和较低的口服生物利用性.
- 需要有效的输送系统来增强黄素的治疗潜力.
研究的目的:
- 阐明植物蛋白质分子特征如何影响黄素载纳米颗粒的组装,结构和消化命运.
- 建立一个结构-功能关系,用于设计基于蛋白质的输送系统,用于疏水生物活性物质.
主要方法:
- 采用pH转移策略,用zein作为疏水的核心,以及可溶性豆类蛋白分或乳清蛋白分离物作为外组件.
- 在模拟的胃条件下,以大小,均性,负载能力,稳定性和黄素保留量为特征的核心外纳米粒子.
- 采用皮体分析和相关性分析来研究蛋白质相互作用及其对纳米粒子消化能力的影响.
主要成果:
- 开发了可调节尺寸 (40-130nm) 的核心外纳米粒子,具有高均性和出色的负载能力 (高达316.3μg/mg蛋白).
- 证明贝蛋白的身份和组成调节了纳米粒子稳定性,黄素封装效率和储存稳定性 (>93%的保留率).
- 展示了优越的胃稳定性和延迟的黄素释放,与ain-mung豆可溶性蛋白 (4MsupZC) 纳米颗粒,在1小时消化后保留75.1%的黄素.
结论:
- 建立了蛋白质分子特征和纳米粒子性能之间的多层结构-功能关系.
- 蛋白质相互作用显著调节纳米粒子组装,稳定性和消化命运,影响黄素的释放.
- 为设计可扩展的基于蛋白质的输送系统提供了机械基础,用于溶解不良的营养药品和药品.
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