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便携式视觉平台集成了聚合酶螺旋放大和CRISPR/Cas12a,用于食品传播细菌的临床检测

Caihong Yin¹, Boyuan Chen¹, Xiaoxi Zheng¹

¹School of Public Health, Jilin University, Changchun, Jilin, 130021, PR China.

Journal of dairy science

|December 15, 2025

概括

一种新的测定方法结合了聚合酶螺旋放大 (PSR) 和CRISPR/Cas12a,以快速检测金黄色葡萄球菌. 这种便携式设备提供了一个灵敏的,低成本的解决方案,用于在资源有限的设置点的护理诊断.

科学领域:

微生物学微生物学
生物技术是生物技术.
公共卫生公共卫生

背景情况:

黄金葡萄球菌 (Staphylococcus aureus) 是一种主要的食源性病原体,造成重大公共卫生问题.
当前的检测方法在资源较少的环境中往往是不切实际的,需要可访问的护理点解决方案.
对于S. aureus的快速,可靠和具有成本效益的诊断非常需要.

研究的目的:

开发一个敏感和特定的检测平台,用于金黄色葡萄球菌.
创建一个便携式,全合一的设备,用于快速的现场检测.
提供适合资源有限环境的多功能诊断工具.

主要方法:

整合聚合酶螺旋放大 (PSR) 以提高灵敏度.
利用CRISPR/Cas12a系统对金黄色细菌进行序列特定的识别.
开发了一种便携式全合一移动检测 (PAMD) 设备用于现场测试.

主要成果:

达到了S. aureus.的10个殖民地形成单位/毫升的检测极限.
使用PAMD的PSR-CRISPR/Cas12a-IOT测定可在60分钟内进行视觉检测.
经过验证的操作方便,低成本和强大的性能,无需复杂的设备.

结论:

开发的PSR-CRISPR/Cas12a-IOT-PAMD平台是低资源设置和现场检测的理想选择.
该测试提供了一种灵敏,快速和具有成本效益的解决方案,用于识别S. aureus.
该平台的多功能性与可互换的原料允许更广泛的应用在传染病诊断.

关键词:

这就是CRISPR/Cas12a系统.食物传播的病原体聚合酶螺旋放大放大便携式移动检测装置,便携式移动检测装置.

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