托尔类受体2的激活揭示了微和纳米塑料上除了内毒素之外的微生物污染
Øyvind P Haugen1, Itziar Polanco-Garriz2, Victor Alcolea-Rodriguez3
1National Institute of Occupational Health (STAMI), Oslo, Norway.
概括
微型和纳米塑料 (MNP) 的研究可能由于微生物污染而存在缺陷. 收费类受体 (TLR) 记者细胞在MNP试验材料中提供了比传统分析更广泛的微生物污染物的检测.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 微生物学 微生物学
背景情况:
- 微塑料和纳米塑料 (MNP) 的健康危害评估经常忽视测试材料中的微生物污染.
- 这种监督可能导致MNP毒性研究的误解,假设原始材料条件.
研究的目的:
- 选MNP试验材料的微生物污染物.
- 为了比较托尔类受体 (TLR) 记者细胞 (TLR2和TLR4) 的有效性,用Limulus amebocyte lysate (LAL) 试验检测微生物配体.
主要方法:
- 八种MNP试验材料被选为微生物污染物.
- 针对TLR2和TLR4的收费类受体 (TLR) 记者细胞被利用.
- 为了进行比较,使用了Limulus amebocyte lysate (LAL) 试验.
主要成果:
- 在8种MNP试验材料中,有5种含有激活TLR2和/或TLR4.4的微生物配体.
- 通过LAL和TLR4测定对内毒素负的MNP仍然可以激活TLR2.
- 基于TLR4的查检测到内毒素污染比LAL测定更有效.
结论:
- 微生物配体在MNP试验材料中普遍存在.
- 与LAL测定相比,TLR记者细胞测定提供了更广泛的微生物污染物的检测.
- 准确的MNP健康危害评估需要考虑微生物污染.
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