通过绿色双酶技术实现Pleurotus pulmonarius的可持续提升:对功能性蛋白质水解酶的新型优化
Nguyen Thi Ngoc Giang1,2, Tran Van Khai3,2
1Experimental-practical Area, An Giang University, Viet Nam.
Food chemistry: X
|December 16, 2025
概括
这项研究开发了一种可持续的双酶水解方法,用于将越南灰加工成富含营养成分的成分. 优化的工艺增强了氨基酸和含量,为功能性食品和调味品提供了有价值的途径.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 可持续化学 可持续化学
背景情况:
- 灰 (Pleurotus pulmonarius) 是越南本土的资源,具有很高的营养潜力.
- 开发可持续的利用方法对于食品行业的创新和减少废物至关重要.
研究的目的:
- 建立可持续的双酶水解方法,从灰中生产营养密集的水解盐.
- 为了优化水解过程,提高回收,氨基酸,,谷氨酸和酸的含量,同时最大限度地减少氨酸的形成.
主要方法:
- 响应表面方法 (RSM) 和人工神经网络-遗传算法 (ANN-GA) 用于过程优化.
- 双酶水解被用来将蛋白分解为生物活性和氨基酸.
- 系统地调查了包括温度,时间和pH值在内的关键参数.
主要成果:
- 与RSM (RMSE 2-5%) 相比,ANN-GA模型显示出更高的预测准确度 (RMSE 1-2%).
- 在最佳的水解条件下 (50.9°C,6.45h,pH6.12) 产生了显著的改善:27.23%的回收,6.926 g/100g氨基酸,11.41 g/100g,6.398 g/100g谷氨酸和0.0845 g/100g.
- 氨的形成被最小化到0.0089g/100g.
结论:
- 开发的双酶水解方法是有效和可持续的,用于生产高价值的水解剂.
- 由此产生的水解酸富含必需氨基酸和,与以前的研究相比或超过.
- 这种方法提供了一个实用的方法,可以从生物质中制造功能性食品成分和调味品,使农民受益并减少浪费.
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