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Updated: Jan 8, 2026

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
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T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

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通过快速缓冲区在线交换-离子移动-质谱测量通过时间解析的细胞内蛋白相互作用和结构动力学

Jun Liu1, Xiaoli Wang1, Xiangjun Si1

  • 1Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Science, Frontiers Science Center for New Organic Matter, College of Chemistry, Nankai University, Tianjin, 300071, China.

Angewandte Chemie (International ed. in English)
|December 16, 2025
PubMed
概括

研究人员开发了RapiBOX-IM-MS,用于实时分析细胞中的蛋白质. 这个新平台揭示了环境压力因素如何触发蛋白质错折和疾病.

关键词:
缓冲在线交易所 在线交易所缓冲数字温度控制器可以控制温度.在细胞内的MSMS.离子移动性质谱法质谱法蛋白质相互作用 蛋白质相互作用

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Published on: April 17, 2017

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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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