相关实验视频

Updated: Jan 8, 2026

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

Published on: June 23, 2018

纳米粒子冠状病毒中的肝素结合蛋白通过糖核相互作用增强细胞吸收

Paulo H Olivieri¹, Jackelinne Y Hayashi¹, Ricardo J S Torquato¹

¹Department of Biochemistry, Federal University of São Paulo (UNIFESP), São Paulo, São Paulo 04044-020, Brazil.

|December 16, 2025

概括

纳米颗粒 (NPs) 与细胞表面的葡萄糖相互作用. 在NP冠状体上的肝素结合蛋白 (HBPs) 通过与葡萄糖接触来增强细胞吸收,从而提高细胞内传递效率.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
细胞生物学细胞生物学

背景情况:

在内部化之前,用于细胞内输送的纳米颗粒 (NP) 面向细胞表面的葡萄糖.
葡萄糖可能起到屏障作用或为NP冠状病毒提供识别点.
了解NP-glycocalyx相互作用对于优化输送至关重要.

研究的目的:

调查糖核在纳米粒子 (NP) 吸收中的双重作用.
确定血蛋白冠状体,特别是肝素结合蛋白 (HBPs),如何影响NP-glycocalyx相互作用和随后的细胞吸收.
通过工程冠状组合来探索增强NP交付的策略.

主要方法:

准备的NP与皇冠丰富或耗尽在HBPs.
使用模型冠冕与选定的HBPs和非HBPs.
生物物理测试以评估肝素相互作用.
细胞吸收研究使用葡萄糖耗尽的细胞和竞争测定与肝素和抗血素.

主要成果:

富含HBP的NP表现出强烈的肝素相互作用,而缺乏HBP的NP则表现出较弱的结合.
特定的HBP (抗血素,阿波利波蛋白E,血小板因子4) 通过葡萄糖相互作用显著增强NP保留和吸收.
缺乏HBP的NP表明相互作用和吸收减少.
从生理上提取的coronas从失脂血清中,富含HBPs,促进了更有效的依赖葡萄糖的NP吸收.

结论:

细胞表面的葡萄糖积极与冠状 HBPs 接触,以驱动高效的 NP 吸收.
葡萄糖的相互作用代表了一种被低估的增强NP交付的机制.
优化NP冠状组合以利用葡萄糖相互作用对于有效的细胞内传递至关重要,而不仅仅是准膜受体.

关键词:

细胞吸收的时间葡萄糖糖的性糖质 (glycocalyx) 是一种肝硫酸盐是什么肝硫酸盐纳米颗粒是一种纳米粒子.蛋白质冠状病毒冠状病毒

更多相关视频

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Published on: January 15, 2018

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Published on: September 2, 2022

相关概念视频

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...

Drug Binding to Blood Components

Drug Binding to Blood Components

When drugs enter systemic circulation, they interact with various components of the blood, including proteins such as human serum albumin (HSA), α1-acid glycoprotein (AAG), lipoproteins, globulins, and red blood cells (RBCs).
HSA is the most abundant plasma protein and is vital in drug binding. It contains distinct drug-binding sites, with different drugs exhibiting affinity for specific sites. There are three main drug-binding domains for HSA: sites I, II, and III. These domains are...

Glycocalyx and its Functions

Glycocalyx and its Functions

The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.