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受精失败试验确定了完全受精失败的夫妇的特定阶段停产

Jing Dai^1,2, Yueren Wu³, Jianfang Che³

¹NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Xiangya School of Basic Medical Sciences, Central South University, Changsha, China.

Molecular human reproduction

|December 16, 2025

概括

现在可以将受精失败分为特定的停止阶段,从而实现个性化治疗. 辅助卵细胞激活可以改善基因组合缺陷的结果,但不能改善前核形成缺陷.

科学领域:

生殖生物学生殖生物学
辅助生殖技术 (ART) 是一种助孕技术.
遗传学是一个遗传学.

背景情况:

受精失败是ART的一个重大挑战,缺乏特定的诊断阶段和个性化策略.
完全受精失败 (TFF) 需要了解有效干预的确切停止点.

研究的目的:

为了对TFF夫妇的受精停止阶段进行分类.
根据停产阶段,比较使用常规ICSI和ICSI辅助卵细胞激活 (ICSI-AOA) 的受精和胚胎率.
确定导致受精失败的遗传因素.

主要方法:

在IVF或ICSI期间对445对经历TFF的夫妇进行了受精失败试验 (FFA).
对比分析评估了ICSI或ICSI-AOA的后续周期中的受精和胚胎质量.
整体外体测序 (WES) 用于研究20对夫妇的遗传突变.

主要成果:

确定了四种受精停止表型:精子透/融合缺陷 (SPD),基因素合并缺陷 (HID),前核形成缺陷 (PFD) 和混合缺陷.
SPD是IVF中TFF的主要原因,由ICSI解决.
在ICSI中,HID和PFD是TFF的原因;ICSI-AOA改善了HID的结果,但不是PFD. 鉴定出了男性和女性病因的遗传因素.

结论:

FFA有效地对受精停止阶段进行了分类,有助于理解受精进展.
根据FFA结果,可以制定个性化治疗策略,包括ICSI-AOA对HID的治疗策略.
识别与特定停产阶段相关的遗传突变,可以了解受精失败机制.

关键词:

美国国务院调查局 (ICSI)辅助卵细胞激活辅助卵细胞激活没有受精失败是受精失败.遗传评估遗传评估遗传评估遗传评估基因组基因组基因组

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