在增强剂内抑制序列的演变有助于十字植物的形态多样性
Alessandro Popoli1, Remco A Mentink1, Lisa Brombach1
1Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne 50829, Germany.
概括
在从LMI1复制后,RCO基因的调控进化涉及压制序列. 这种负面调节塑造了RCO表达,为复杂的叶子进化做出了贡献,并防止了类效应.
科学领域:
- 发展生物学 发展生物学
- 进化遗传学 进化遗传学
- 分子生物学分子生物学
背景情况:
- 像增强剂这样的Cis调节序列对于形态进化至关重要.
- 了解增强多元化如何推动新的表达模式和多样性是具有挑战性的.
- 基因组序列背景显著影响增强器功能.
研究的目的:
- 研究RCO本体盒基因的调节,这对复杂的叶子进化至关重要,在从LMI1.1开始的协同重复后,该基因对复杂的叶子进化至关重要.
- 描述LMI1/RCO位点的调节性等位体,以了解增强剂的演变.
- 确定负调节在塑造RCO表达域中的作用.
主要方法:
- 基因组编辑以在LMI1/RCO位点产生17个调节性等位基因.
- 通过删除和记者基因测试来表征增强器功能.
- 在RCO增强器中映射正负调节序列.
主要成果:
- 进化的RCO增强器表现出明显的负调节,划分其表达域.
- 删除RCO增强剂只导致部分功能丧失,与LMI1增强剂不同.
- 在RCO增强器中发现了一种与嵌套重复相关的抑制序列.
- 这种压制序列对于定义RCO表达域和防止类型变异至关重要.
结论:
- 压制序列的进化是监管进化的重要机制.
- 增强剂内的负调节在塑造形态新奇性的特定表达模式方面发挥着关键作用.
- 了解强化器的监管特征,包括压制,对于解释进化多样化至关重要.
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