来自2型糖尿病大鼠的胆汁加工科普蒂迪斯根茎瘤的柏柏林的比较药理动力学和组织分布
Ying Zhu1, Lu Yang1, Qinwan Huang2
1College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Chemistry & biodiversity
|December 16, 2025
概括
与传统加工相比,胆处理的Coptidis Rhizoma (BPCR) 提高了2型糖尿病 (T2DM) 鼠的柏柏林水平和组织分布. 猪胆汁 (PB) 处理对Coptidis Rhizoma (CR) 在T2DM治疗中的药理动力学和疗效产生重大影响.
科学领域:
- 药理学和药理动力学 药理学和药理动力学
- 传统中国医药 传统中国医药
- 代谢障碍 代谢障碍 代谢障碍
背景情况:
- 与单独的Coptidis Rhizoma (CR) 相比,胆处理的Coptidis Rhizoma (BPCR) 在2型糖尿病 (T2DM) 中表现出更高的疗效.
- 猪胆汁 (PB) 处理对CR中活性化合物的药理动力学概况的特定影响仍然在很大程度上未被描述.
- 了解这些药理动力学变化对于阐明BPCR在T2DM中增强的治疗机制至关重要.
研究的目的:
- 在T2DM的老鼠模型中研究BPCR的药理动力学和组织分布.
- 在口服后在血清中识别BPCR的化学成分和代谢物.
- 阐明在BPCR中归因于PB处理的增强机制.
主要方法:
- 建立了一个T2DM大鼠模型,使用高脂肪饮食和链毒素诱导.
- 超高性能液态染色学四极管轨道高分辨率质谱法 (UHPLC-Q-Orbitrap HRMS) 用于血清成分的识别.
- 超高性能液态染色学-并联质谱学 (UHPLC-QQQ-MS/MS) 量化了不同CR处理组 (CR,炸CR,BPCR) 的血和组织中的柏柏林含量.
主要成果:
- 血清分析确定了贝贝林作为主要原始化合物,以及四种代谢物,在BPCR给药后.
- 在T2DM大鼠中,与CR和FCR相比,BPCR的使用导致药物动力学参数 (Cmax,Tmax,HL_Lambda_z) 显著改变 (p <0.05).
- 血和各种组织中的柏柏林度在BCCR给药后与CR和FCR相比始终更高,在T2DM大鼠和对照群中观察到显著增加的水平 (p <0.05).
结论:
- 猪胆汁 (PB) 处理显著改变了来自Coptidis Rhizoma (CR) 的柏柏林的药理学行为和组织分布.
- 在用BPCR治疗的T2DM大鼠中,柏柏林的增强吸收和组织分布可能有助于其改善的治疗疗效.
- 这项研究提供了对BPCR增强抗糖尿病作用的机制的关键见解,强调了传统加工方法的重要性.
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