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模拟CeO₂纳米酶的aptamer调节的氧化酶,用于多色和比度色度感测

Gengli Huang¹, Shuo Qi², Xiaodong Lu¹

¹State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, 214122, PR China; Key Laboratory of Screening, Prevention, and Control of Food Safety Risks, State Administration for Market Regulation, Wuxi, Jiangsu, 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, PR China.

|December 16, 2025

概括

一个新的多彩的感应传感器使用氧化 (CeO2) 纳米酶与氧化酶 (HPO) 模仿活动的敏感,可视检测kanamycin. 这个平台提供了增强的比度传感,用于现场监测食品安全.

科学领域:

* 纳米材料科学科学纳米材料科学
* 分析化学分析化学
* 生物感应生物感应

背景情况:

*色度纳米传感器在视觉解释方面经常面临挑战,原因是颜色的微妙变化.
*现有的单次吸收峰值传感器缺乏精确量化的灵敏度和清晰度.
* 开发强大的多色传感平台对于准确的视觉检测至关重要.

研究的目的:

* 开发使用具有模仿氧化酶 (HPO) 活性的CeO2纳米酶的多色光感应传感器.
* 为了实现比度色度和视觉传感,以提高检测灵敏度.
* 创建一个现场食品安全监测平台,特别是用于检测抗生素残留物.

主要方法:

* 整合CeO2纳米酶与HPO模仿活性用于双吸收峰值变化.
* 适合aptamer调节的酶抑制策略,用于调节纳米酶活性.
*比度信号分析 (ΔA620/A430) 和基于智能手机的RGB量化.
* 用牛奶和蜂蜜矩阵进行验证,用于真实样本分析.

主要成果:

* CeO2纳米酶平台通过430nm和620nm的双吸收峰值诱导了明显的黄色到蓝色色调变化.
* 通过aptamer调节的调制导致颜色从蓝色转变为黄色,从而使视觉区分.
*与单波长检测相比,放射测量检测显示了超过6倍的灵敏度改善.
* 在牛奶和蜂蜜样本中实现了可靠的回收,证明了实际效用.

结论:

*开发的HPO纳米酶平台能够实现先进的多色比度生物传感.
*这种方法显著提高了目标检测的灵敏度和视觉清晰度.
* 胃感应器在现场监测食品安全和检测抗生素残留物方面具有广泛的适用性.

关键词:

亚普特美尔 (Aptamer) 是一种药物.这是一种类似氧化酶的氧化酶.纳米酶纳米酶是一种纳米酶.比率测量色度测量方法比率测量色度测量方法视觉检测视觉检测是一种视觉检测.

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