通过综合生物信息学分析识别细胞衰老和支气管喘中的潜在关键基因
Lingyu Pan1, Yeke Jiang2, Dezhi Yuan3
1The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, 230031, Anhui, China; Anhui Hospital of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Hefei, 230031, Anhui, China.
Biochemical and biophysical research communications
|December 16, 2025
概括
细胞衰老与支气管喘 (BA) 有关. 这项研究确定了参与喘和细胞衰老的关键基因 (CBX6,EP300,NFE2L2,KMT2A),这表明了BA治疗的潜在治疗标.
科学领域:
- 基因组学和生物信息学
- 细胞生物学 细胞生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 细胞衰老越来越被认为是发展支气管喘 (BA) 的重要因素.
- 通过了解细胞衰老的作用来确定BA的新型治疗点,对于有效的治疗策略至关重要.
研究的目的:
- 通过分析基因表达数据,研究细胞衰老和支气管喘 (BA) 之间的关系.
- 确定参与BA细胞衰老的关键基因和途径,并探索它们作为治疗点的潜力.
- 评估凯平颗粒 (KCP) 对BA.这些已识别的基因和细胞衰老标志物的调节作用.
主要方法:
- 从基因表达综合 (GEO) 数据库下载并分析了BA相关的基因表达数据集 (GSE143303,GSE147878).
- 采用差异基因表达 (DEG) 分析,权重基因共同表达网络分析 (WGCNA) 和功能丰富分析.
- 集成的与衰老相关的基因组与BA数据用于识别交叉基因,其次是蛋白质-蛋白质相互作用分析,ROC曲线,GSEA和西方斑点验证.
主要成果:
- 在BA中确定了580个差异表达基因 (DEG),CBX6,EP300,NFE2L2和KMT2A被确定为中央调节基因.
- 在BA大鼠模型中证实细胞衰老标志物 (p16,p21) 的水平增加,表明细胞衰老.
- 在BA大鼠中观察到枢纽基因 (CBX6,EP300,NFE2L2,KMT2A) 的变异表达,而Kechuanping颗粒 (KCP) 显示出调节作用.
结论:
- 凯川平颗粒 (KCP) 在喘中的治疗效果可能归因于其调节呼吸道光滑肌细胞 (ASMC) 衰老.
- 通过调节细胞衰老,CBX6,EP300,NFE2L2和KMT2A被确定为参与KCP抗喘作用的关键分子标.
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