相关实验视频

Updated: Jan 8, 2026

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

一个染色体水平的参考基因组组装的巨型 (Pangasius sanitwongsei)

Baojiang Gan¹, Lingjing Wei¹, Yuanxiong Ma¹

¹Aquatic Species Introduction and Breeding Center of Guangxi, Nanning, 530031, China.

Scientific data

|December 16, 2025

概括

研究人员已经测序了危巨型鱼 (Pangasius sanitwongsei) 的基因组,这是一种重要的淡水鱼. 这种高质量的基因组组件对于了解其遗传适应性和制定有效的保护策略至关重要.

科学领域:

基因组学就是基因组学.
保护生物学保护生物学
水产养殖是水产养殖的一种方式.

背景情况:

巨型鱼 (Pangasius sanitwongsei) 是一个临灭绝的淡水鱼类.
它在东南亚和中国南部具有重要的生态和经济意义.
有限的基因组数据阻碍了对其遗传适应和保护的研究.

研究的目的:

为了生成一个高质量的,染色体规模的基因组组合,巨型.
为保护基因组学和理解适应性进化提供基础资源.
支持该物种的可持续水产养殖发展.

主要方法:

使用了PacBio HiFi长读测序技术.
在基因组组装中使用了Hi-C染色体构造捕获.
对基因注释和功能表征进行了生物信息分析.

主要成果:

成功生成了808.85 Mb的染色体规模基因组组.
该组件实现了高连续性 (N50:18.70 Mb) 和脚手架完整性 (N50:28.10 Mb).
报告了高的BUSCO基因完整性 (99.15%) 和广泛的基因注释 (23,469个基因).

结论:

高质量的基因组组合为Pangasiidae鱼的适应性进化提供了关键的见解.
这种基因组资源对于未来的保护基因组学,种群遗传学和适应性进化研究是无价的.
这些发现将有助于制定有效的保护策略和可持续的水产养殖实践,以保护巨型.

更多相关视频

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...