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Updated: Jan 8, 2026

Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss

Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss

Published on: July 19, 2024

来自TOTUM-448 (植物制剂) 的可生物利用的人类代谢物在高脂环境中维持肝细胞功能,这推动了MASLD的发病

Fabien Wauquier¹, Vivien Chavanelle², Annie Bouchard-Mercier²

¹Faculté de Médecine, Clinic'n'Cell SAS, 28 place Henri Dunant, Clermont-Ferrand, 63001, France.

Scientific reports

|December 16, 2025

概括

来自TOTUM-448的植物性多代谢物保护肝细胞免受脂肪积累和炎症的影响. 这项研究显示了营养干预措施在管理代谢功能障碍相关的脂肪性肝病 (MASLD) 的潜力.

科学领域:

肝病学肝病学是一种肝病学.
营养科学营养科学
生物化学生物化学

背景情况:

与代谢功能障碍相关的脂肪性肝病 (MASLD) 是由脂毒性和炎症驱动的.
目前的MASLD治疗有副作用,需要使用营养干预等替代策略.
预防性营养方法需要进一步临床验证其有效性.

研究的目的:

调查丰富多的配方TOTUM-448生物可用的代谢物对肝细胞功能的影响.
评估这些代谢物如何在体外影响MASLD的关键病理特征.
探索植物性化合物在肝脏健康中的潜在治疗机制.

主要方法:

在TOTUM-448摄入后,UHPLC-MS/MS分析以识别和描述人血清中的多代谢物.
用代谢物丰富血清治疗HepG2肝细胞,有或没有Palmitate的挑战.
评价细胞活力,脂质代谢,炎症,氧化应激和内质网膜 (ER) 应激标志物.

主要成果:

TOTUM-448代谢物对棕酸盐诱导的脂毒性产生了耐药性,减少了细胞内脂质的积累.
代谢物抑制了palmitate诱导的炎症基因表达的上调.
TOTUM-448的代谢物正常化了ER压力标志物 (CHOP,XBP1,ATF6,Caspase-3) 通过棕酸盐升高.

结论:

来自TOTUM-448的循环代谢物保护肝细胞免受脂毒性和炎症性压力.
这些发现支持TOTUM-448作为MASLD的预防性营养策略的潜力.
需要进一步的临床研究来证实这种植物性配方的治疗益处.

关键词:

临床试验临床试验是指临床试验的临床试验.活体外 (ex vivo) 生物体外.人类肝细胞是人类肝细胞.人类代谢物的人类代谢物.脂毒性压力是一种压力.马斯尔德马斯尔德是什么意思氧化应激是一种氧化应激.植物提取物是一种植物提取物.

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