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肠道微生物群和宿主转录组相互作用揭示了MASLD相关的肝细胞癌中的诊断生物标志物

Thananya Jinato^1,2, Jakkrit Khamjerm¹, Satjapot Manprasong³

¹Center of Excellence in Hepatitis and Liver Cancer, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.

|December 16, 2025

概括

肠道微生物群影响了与代谢功能障碍相关的脂肪性肝病 (MASLD) 和肝癌 (HCC). 特定的肠道细菌和宿主基因,如COL10A1,显示出作为MASLD-HCC早期诊断标记物的潜力.

科学领域:

胃肠病学和肝病学
微生物组研究微生物组研究
在瘤学瘤学.

背景情况:

肠道微生物组的改变与代谢功能障碍相关的脂肪性肝病 (MASLD) 和肝细胞癌 (HCC) 有关.
在MASLD和HCC中,肠道细菌与宿主基因表达之间的相互作用尚未得到充分理解.

研究的目的:

调查肠道微生物组组成和MASLD和HCC.宿主基因表达之间的关系.
确定潜在的诊断生物标志物,用于早期检测与MASLD相关的HCC.

主要方法:

对便肠道微生物组和外围血液单核细胞 (PBMC) 的转录基因分析.
综合分析以识别差异表达基因 (DEG) 和细菌种群.
潜在生物标志物的实时PCR验证.

主要成果:

260个DEG和29个细菌种群区分了没有HCC的MASLD和MASLD-HCC.
七个细菌系与84个宿主基因相关,其中*Veillonella*与RIMS3,COL10A1和ENAH相关.
作为MASLD与MASLD-HCC (AUC=0.835) 的生物标志物,COL10A1得到了验证;与AFP或*Veillonella*的组合改善了分化 (AUC=0.941-0.996).

结论:

肠道微生物组与宿主基因相互作用在与MASLD相关的HCC进展中具有重要意义.
特定的细菌属和宿主基因表达特征可以作为MASLD-HCC的早期诊断标记物.

关键词:

我们的肠道微生物群.在HCC中,HCC是HCC.宿主基因是指宿主基因的基因.马斯尔德马斯尔德是什么意思马斯尔德-HCCCC公司转录的个人资料.

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