相关实验视频

Updated: Jan 8, 2026

Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

Published on: January 27, 2023

来自人类肝脏原始细胞的生物完整的治疗外体,用于肝脏疾病

Taewoon Kim¹, Gul Karima¹, Luke P Lee^2,3,4

¹Department of Bionanotechnology, Graduate School, Hanyang University, Seoul, Republic of Korea.

Advanced healthcare materials

|December 17, 2025

概括

在代谢功能障碍相关的脂肪性肝病 (MASLD) 和酒精相关的肝病 (ALD) 模型中,生物完整的外体细胞有效地减少肝脂. 这种无细胞疗法在治疗这些普遍的肝病方面表现有前途.

科学领域:

肝病学肝病学是一种肝病学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

与代谢功能障碍相关的脂肪性肝病 (MASLD) 和与酒精相关的肝病 (ALD) 在全球范围内正在增加.
目前对MASLD和ALD的治疗方法有限,基于外体的疗法面临生物完整性的挑战.

研究的目的:

为了研究生物完整的外体的治疗潜力,用于治疗脂肪性肝病.
在MASLD和ALD模型中评估外体在减少脂质积累方面的有效性.

主要方法:

使用BEST技术分离了生物学上完整的外体 (30-200nm),并通过CD63和CD81标记物证实了这一点.
用油酸和乙醇在AML12细胞中创建了性肝病模型,保持高细胞活力.
含有hsa-miR-122-5p和hsa-miR-27a-3p的外基因组被给予疾病模型.

主要成果:

完整的外体细胞在MASLD模型中显著降低了脂质积累,高达92.7%,在ALD模型中降低了93.2%.
治疗在72小时内100μg/ml完整外体的剂量有效.
与正常肝细胞相比,该方法维持了大约90%的细胞活力.

结论:

在生物学上完好无损的外体体显示出显著的治疗效果,减少肝硬化症.
基于外体细胞的无细胞疗法有可能逆转MASLD和ALD.
这种方法为治疗流行性肝病提供了一个有希望的策略.

关键词:

外基因组是外基因组中的一个.肝脏的原始细胞是肝脏的原始细胞.脂质新陈代谢脂质新陈代谢肝脏疾病是一种肝脏疾病.治疗药物治疗药物

更多相关视频

Generation of Functional Endodermal Hepatic Organoids

Generation of Functional Endodermal Hepatic Organoids

Published on: May 2, 2025

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Published on: May 10, 2020

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...