相关实验视频

Updated: Jan 8, 2026

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

益生菌DNA在表观遗传学上调节KDM4A降解,以减轻气道过敏

Huanping Zhang¹, Xiaoyao Li², Mingxian Zhang²

¹Department of Allergy Medicine, Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan 030032, China.

|December 17, 2025

概括

益生菌DNA通过上调USP14和KDM4A.A的调节,恢复了气道过敏 (AA) 的小鼠的1型调节性T细胞 (Tr1) 功能. 这种机制针对Tr1功能障碍,为AA提供了潜在的治疗策略.

科学领域:

免疫学免疫学免疫学
过敏研究研究过敏
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

1型调节性T细胞 (Tr1) 功能障碍是气道过敏 (AA) 病原体的核心.
在AA中底层的Tr1功能障碍的精确分子机制在很大程度上是未知的.

研究的目的:

在AA的小鼠模型中研究益生菌DNA在恢复Tr1细胞功能中的作用.
探索USP14和KDM4A在益生菌DNA介导的Tr1恢复和AA缓解中的参与.

主要方法:

使用灰尘虫提取物开发AA小鼠模型.
通过流式细胞计量评估Tr1细胞功能和Th2细胞极化.
通过使用ChIP和ubiquitin免疫阻塞,分析KDM4A,USP14和Il-10促进体表观遗传学.

主要成果:

AA小鼠表现出Tr1免疫抑制受损,KDM4A在Il-10促进剂减少,Il-10促进剂高甲基化增加 (H3K9me3),USP14.
CD4+ T 细胞中的 KDM4A 缺乏导致了自发的 Th2 气道极化和类似 AA 的病理.
益生菌DNA给药提高了USP14,恢复了KDM4A水平,增强了Tr1功能,并改善了小鼠的AA反应.

结论:

减少与Il-10促进体相关的KDM4A活性是AA中Tr1细胞功能障碍的关键驱动因素.
益生菌DNA通过上调USP14来减轻AA,从而恢复KDM4A功能和Tr1细胞的免疫抑制能力.
USP14和KDM4A代表了管理气道过敏的潜在治疗点.

关键词:

卫生科学卫生科学免疫反应的免疫反应.免疫学免疫学免疫学分子生物学分子生物学

更多相关视频

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Published on: September 26, 2015

相关概念视频

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...