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突触记忆:用蛋白质条形码映射神经元的单个突触

Carsten H Tischbirek^1,2, Linjing Fang¹, Duncan M Chadly¹

¹Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

bioRxiv : the preprint server for biology

|December 17, 2025

概括

突触记忆 (Synaptic MEMOIR) 是一种用于绘制单突触分辨率的神经回路的新方法. 这种光学成像技术克服了连接学中的分辨率-体积权衡,使大规模的大脑布线图重建成为可能.

科学领域:

神经科学是一个神经科学.
在Connectomics上,我们提供了连接.
分子生物学分子生物学

背景情况:

对神经回路 (连接学) 的绘制对理解大脑功能至关重要.
像电子显微镜这样的当前方法面临着分辨率-体积的权衡,限制了可扩展性.
追踪长距离的神经元投影是复杂的,容易出错.

研究的目的:

开发一种可扩展的光学成像方法,用于重建神经线路图.
在大脑体积中实现单突触分辨率映射.
克服现有的连接技术的局限性.

主要方法:

突触记忆 (Synaptic MEMOIR) (记忆与工程变异与光学 in situ 读取) 使用蛋白质条形码传输到突触.
持续的突变发生产生各种条形码,用于独特的细胞体和突触标签.
300nm分辨率的光学显微镜用于条形码识别,避免高分辨率的跟踪.

主要成果:

在 *Drosophila melanogaster* 光叶 (9,500万μm3) 中展示了113个神经元的投影映射.
突触条形码与细胞体条形码相匹配,没有高分辨率成像.
该方法允许跨大脑部分进行条形码匹配,并且不需要组织清除.

结论:

交互记忆 (Synaptic MEMOIR) 提供了一个可扩展的光学成像基础,用于神经线路图的重建.
该技术可以在不同的发育阶段,遗传背景和扰乱中应用.
这种方法有可能扩展到与小鼠大脑图谱相美的体积.

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