在Drosophila的tRNA剧目演变过程中,性别偏差的重复被迅速生成
Dylan Sosa1, Marek Sobczyk2, Jianhai Chen1
1Department of Ecology & Evolution, University of Chicago, Chicago, IL, U.S.A.
bioRxiv : the preprint server for biology
|December 17, 2025
概括
转移RNA (tRNA) 基因进化在果中显示持续的收益和损失,导致独特的副本和性二态表达. 这表明tRNA基因动态是由性和自然选择塑造的.
科学领域:
- 进化生物学 进化生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 细胞基因组含有众多转移RNA (tRNA) 基因,被认为可以优化翻译.
- 进化机制和选择压力塑造的tRNA基因家族在metazoans由于测序的挑战是不太了解.
研究的目的:
- 调查drosophilid物种转移RNA (tRNA) 基因库的进化史和多样化.
- 探索tRNA基因进化的功能和调控影响,包括密码子的使用和表达模式.
主要方法:
- 利用从24个Drosophilid物种的长时间读取的基因组组件来重建tRNA基因起源时间.
- 在 *Drosophila melanogaster* 中进行了tRNA测序,以分析最近重复的tRNA基因的表达,碎片化和修改模式.
主要成果:
- 在6000万年间,确定了tRNA基因重复的连续增加和损失,导致异位编码和异位接受副本的快速分类学限制.
- 发现了与翻译效率不一致的编码器使用模式,并确定了一个特定于D. melanogaster的异码编码器.
- 在分类学上受限制的tRNA副本中观察到的性二态表达,碎片化和核基基改性.
结论:
- 分类学上受限制的tRNA基因成为监管多样性的重要来源.
- 性选择和自然选择都在塑造tRNA基因家族的进化轨迹中发挥着至关重要的作用.
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