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精确的物种识别和全基因组测序分析Enterobacter cloacae复合体,导致中国的血液感染

Yanbing Li^1,2, Ziran Wang¹, Ge Zhang¹

¹Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Microbiology spectrum

|December 17, 2025

概括

在医院感染中,Enterobacter cloacae复合体 (ECC) 显示出高度的遗传多样性. 肠杆菌 roggenkampii 菌株对碳烯和胆素具有显著的耐药性,需要基于基因组的监测.

科学领域:

微生物学微生物学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

肠杆菌菌综合体 (ECC) 是医院获得感染的重要原因之一,特别是血液感染.
在临床环境中,精确的物种识别和ECC中的耐药性概况具有挑战性.
对于ECC中特定物种的抗菌素耐药性模式的了解有限.

研究的目的:

从血液感染中分离出ECC的综合基因组特征进行.
为了确定物种多样性,抗微生物耐药性基因和ECC分离物体内的基因组变异.
了解物种特异性耐药性模式和ECC在宿主体内的潜在进化.

主要方法:

在2015-2020年期间收集的108个ECC分离物的全基因组测序 (WGS).
使用平均核酸标识 (ANI) 进行物种识别.
对抗微生物耐药性基因的分析,多位置序列类型 (MLST) 和基于hsp60序列的遗传学分析.

主要成果:

确定了11个hsp60集群,其中集群VIII是最普遍的.
百分之31.5的 Enterobacter hormaechei 和百分之15.7的 Enterobacter xiangfangensis 是主要的物种.
20个分离物体对碳烯具有耐药性,其中Enterobacter roggenkampii是最常见的物种 (5/20);83.3%的这些耐药性分离物体对碳烯和胆素都表现出耐药性.
在90个检测到的ST中,发现了29种新型序列类型 (ST).
对比基因组学揭示了纵向隔离物中的pco基因中的适应性SNP.

结论:

这项研究提供了详细的基因组洞察力ECC血流感染孤立在中国北部.
观察到高物种多样性和明显的耐药性基因分布模式.
恩特罗巴克特 roggenkampii被突出显示为一个潜在的高风险物种,与卡巴因和胆素耐药性有关.
建议基于基因组的监测用于管理ECC感染并了解耐药性演变.

关键词:

杆菌cloacae复杂的杆菌.血流感染感染感染进行全基因组测序.

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